Mitochondrial Regulation

Methods and Protocols

AvCarlos M. Palmeira,Anabela P. Rolo

Inbunden, Engelska, 2021

Del i serien Methods in Molecular Biology

2 328 kr

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Beskrivning

The book integrates mitochondria with other cellular components, discussing the dynamic properties of mitochondria with an emphasis on how these processes respond to signaling events and how they affect cellular metabolism.

Produktinformation

Utgivningsdatum:2021-06-08
Mått:178 x 254 x 24 mm
Vikt:823 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:318
Upplaga:2
Förlag:Springer-Verlag New York Inc.
ISBN:9781071614327

Utforska kategorier

Innehållsförteckning

Isolation of Mitochondria from Liver and Extraction of Total RNA and Protein: Analyses of miRNA and Protein Expression.- Determination of Oxidative Phosphorylation Complexes Activities.- BN-PAGE-Based Approach to Study Thyroid Hormones and Mitochondrial Function.- Monitoring Mitochondrial Function in Mouse Embryonic Stem Cells (mESCs).- Mitochondrial Functional Assessment in Mammalian Gametes Using Fluorescent Probes.- Update on the Histoenzymatic Methods for Visualization of the Activity of Individual Mitochondrial Respiratory Chain Complexes in the Human Frozen Sections.- An Update on Isolation of Functional Mitochondria from Cell for Bioenergetics Studies.- PCR-Based Determination of Mitochondrial DNA Copy Number in Multiple Species.- Methods to Monitor Mitophagy and Mitochondrial Quality: Implications in Cancer, Neurodegeneration, and Cardiovascular Diseases.- Analysis of Pro-Apoptotic Protein Trafficking to and from Mitochondria.- Exploring Liver Mitochondrial Function by 13C-Stable Isotope Breath Tests: Implications in Clinical Biochemistry.- Protocols for Mitochondria as the Target of Pharmacological Therapy in the Context of Nonalcoholic Fatty Liver Disease (NAFLD).- The Use of Reactive Oxygen Species Production by Succinate-Driven Reverse Electron Flow as an Index of Complex 1 Activity in Isolated Brown Adipose Tissue Mitochondria.- Mitochondrial Regulation Assessment by 13C-NMR Isotopomer Analysis.- Assays for Determination of Cellular and Mitochondrial NAD+ and NADH Content.- Following the Dynamism of the Mitochondrial Network in T Cells.- Measuring PGC-1α and Its Acetylation Status in Mouse Primary Myotubes.