HIV Protocols

Häftad, Engelska, 2025

Del 2807 i serien Methods in Molecular Biology

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Beskrivning

This fourth edition volume expands on the previous editions with discussions on the latest methodologies to study HIV, live cell imaging, HIV cure, new modifications to the viral RNA that impacts HIV biology, and new types of intracellular compartments.

Produktinformation

Utgivningsdatum:2025-05-15
Mått:178 x 254 x 21 mm
Vikt:699 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:358
Upplaga:4
Förlag:Springer-Verlag New York Inc.
ISBN:9781071638644

Utforska kategorier

Onkologi inom Medicin

Innehållsförteckning

Infectious Virus Tracking by Fluorescent Live Cell Imaging in Primary Cells.- Imaging HIV-1 Nuclear Import, Uncoating, and Proviral Transcription.- RNA-FISH for HIV Transcription/Localization Analysis.- Single-Cell and Single-Molecule RNA-FISH Combined with Immunofluorescence and High-Speed and High-Resolution Scanning Analysis to Visualize Reactivation of Latent HIV-1.- Visualizing HIV-1 Assembly at the T Cell Plasma Membrane using Single Molecule Localization: TIRF Microscopy.- Single-Virion Analysis: A Method to Visualize HIV-1 Particle Content Using Fluorescence Microscopy.- Correlative Imaging to Detect Rare HIV Reservoirs and Associated Damage in Tissues.- Characterization of Nuclear HIV-Induced Membraneless Organelles through Fluorescence Microscopy.- Detection of CPSF6 in Biomolecular Condensates as a Reporter of Nuclear Import.- Monitoring HIV-1 Nuclear Import Kinetics Using a Chemically Induced Nuclear Pore Blockade Assay.- Biochemical Detection of Capsid in the Nucleus During HIV-1 Infection.- Chromatin Immunoprecipitation of Retroviral Genomes with Antibodies Recognizing Modified Histones and Specific Viral Proteins.- Measurement of HIV Rev-Rev Response Element Functional Activity.- Method for the Enrichment of N6-Methyladenosine-Modified Cellular and HIV-1 RNA.- Direct Analysis of HIV mRNA m6A Methylation by Nanopore Sequencing.- Identifying Individual Pseudouridine (Ψ) Sites Across Transcripts from HIV-1 Infected Cells.- Proximity Ligation Assay to Detect the Proximity between Host Proteins and Viral Proteins of HIV-1.- Modeling HIV-1 Infection in CNS via Infected Monocytes using Immunocompetent Brain Organoids.- An In Vitro Model of Blood Brain Barrier for Studies on HIV Neuroinflammation and CNS Antibody Penetration.- Generation of Anti-HIV CAR-T Cells for Preclinical Research.- Highly Sensitive Analysis of Cervical Mucosal HIV-1 Infection using Reporter Viruses Expressing Secreted Nanoluciferase.- Multiparameter Flow Cytometry Monitoring of T Cell Responses.- An Ultrasensitive p24 Assay to Measure HIV-1 in Diverse Biological Matrixes.