Adipogenesis

Methods and Protocols

AvMichael S. Pepper,Melvin A. Ambele

Inbunden, Engelska, 2025

Del 2938 i serien Methods in Molecular Biology

2 267 kr

Beställningsvara. Skickas inom 10-15 vardagar. Fri frakt över 249 kr.

Fler format och utgåvor

E-bok

2 948 kr

Häftad

1 589 kr

Beskrivning

This volume details protocols in Adipogensis research, such as stem cell line isolation, gene expression, obesity research, and drug development. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Adipogenesis: Methods and Protocols aims to be comprehensive guide for researchers in the field.

Produktinformation

Utgivningsdatum:2025-05-31
Mått:178 x 254 x 17 mm
Vikt:582 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:189
Förlag:Springer-Verlag New York Inc.
ISBN:9781071646069

Utforska kategorier

Onkologi inom Medicin

Innehållsförteckning

Adipogenic differentiation from human embryonic stem cells through formation of embryoid bodies.- Quantification of adipogenesis using flow cytometry.- Isolation of Wharton’s Jelly derived Mesenchymal Stromal/Stem Cells.- Adipogenic differentiation assay for the study of insulin signalling.- Quantification of lipid droplets in adipocytes.- RT-qPCR quantification of adipogenic marker genes (Pparg, C/ebpa, Fabp4 and Cd36).- Adipogenic differentiation of human adipose-derived mesenchymal stromal/stem cells (ASCs).- Western blot detection of adipogenic protein.- Measurement of intracellular Reactive Oxygen Species during adipogenesis.- Oxygen consumption and extra cellular acidification rate in adipogenic differentiated human mesenchymal stromal/stem cells.- In vitro expansion of human adipose-derived stem cells (ASCs).- siRNA silencing of adipogenic gene in ASCs.- Freezing and thawing of Adipose-derived stromal/stem cells (ASCs).- Isolation and Characterization of Mouse Adipose-Derived Stromal/Stem Cells.- Isolation of adipose-derived mesenchymal stromal/stem cells.- Isolation and purification of adipocyte-derived extracellular vesicles (AdEVs) using size-exclusion chromatography.- Differentiation of Adipose derived stromal/stem cells into Osteocytes.- Differentiation of mesenchymal stromal/stem cells from induced pluripotent stem cells.- Isolation and Characterization of Equine Mezenchymal Stromal/Stem Cell.

Mer från samma serie

Del 2834

Computational Toxicology

Orazio Nicolotti

Inbunden, 2024

2 041 kr

Del 2996

Biofuels from Algae

Kristian Spilling

Inbunden, 2026

2 202 kr

Adenovirus

Miguel Chillón, Assumpció Bosch

Häftad, 2016

1 191 kr

Ctenophores

Leonid L. Moroz

Häftad, 2025

1 982 kr

Del 2588

Oral Biology

Gregory J. Seymour, Mary P. Cullinan, Nicholas C.K. Heng, Paul R. Cooper

Häftad, 2023

2 267 kr

Proteins

John M. Walker

Inbunden, 1984

1 104 kr

Nucleic Acids

John M. Walker

Inbunden, 1984

1 137 kr

Proteins

John M. Walker

Häftad, 1984

1 104 kr

Nucleic Acids

John M. Walker

Häftad, 1984

1 104 kr

New Protein Techniques

John M. Walker

Inbunden, 1988

1 104 kr

Du kanske också är intresserad av

10% rabatt på allt

Adventskalender Ravensburger pussel 2026

Ravensburger

319 kr

Del 2938

Adipogenesis

Michael S. Pepper, Melvin A. Ambele

Häftad, 2026

1 589 kr

Del 2938

So Pots of Central Africa

Graham Connah

Häftad, 2019

435 kr

Del 2938

Emerging Trends in Data Science, Information and Knowledge Engineering

Robert Stahlbock, Gary M. Weiss, Hamid R. Arabnia, Leonidas Deligiannidis

Häftad, 2026

1 022 kr

Del 2938

Marktorientierte Steuerung Der Gemeinkosten Im Rahmen Des Target Costing

Ralf Sauter

Häftad, 2002

593 kr

Adipogenesis

Melvin A. Ambele, Michael S. Pepper

E-bok

2025

2 948 kr

Del 2938

Untersuchung des Einflusses von Gesteinssplitten auf die Helligkeit von bituminösen Fahrbahndecken

Werner Leins

Häftad, 1980

570 kr

Del 2938

Rechtsprobleme Der Privaten Arbeitsvermittlung Im Berufssport

Hans-Gert Bovelett

Häftad, 2000

543 kr

10% rabatt på allt

Del 11

Medusa

Lars Kepler

Inbunden, 2026

269 kr

10% rabatt på allt

Jag såg hur vi var stjärnor

Laleh Pourkarim

Inbunden, 2026

269 kr

Adipogenesis

Methods and Protocols

AvMichael S. Pepper,Melvin A. Ambele

Inbunden, Engelska, 2025

Del 2938 i serien Methods in Molecular Biology

2 267 kr

Beställningsvara. Skickas inom 10-15 vardagar. Fri frakt över 249 kr.

Fler format och utgåvor

E-bok

2 948 kr

Häftad

1 589 kr

Beskrivning

Produktinformation

Utgivningsdatum:2025-05-31
Mått:178 x 254 x 17 mm
Vikt:582 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:189
Förlag:Springer-Verlag New York Inc.
ISBN:9781071646069

Utforska kategorier

Onkologi inom Medicin

Innehållsförteckning

Adipogenic differentiation from human embryonic stem cells through formation of embryoid bodies.- Quantification of adipogenesis using flow cytometry.- Isolation of Wharton’s Jelly derived Mesenchymal Stromal/Stem Cells.- Adipogenic differentiation assay for the study of insulin signalling.- Quantification of lipid droplets in adipocytes.- RT-qPCR quantification of adipogenic marker genes (Pparg, C/ebpa, Fabp4 and Cd36).- Adipogenic differentiation of human adipose-derived mesenchymal stromal/stem cells (ASCs).- Western blot detection of adipogenic protein.- Measurement of intracellular Reactive Oxygen Species during adipogenesis.- Oxygen consumption and extra cellular acidification rate in adipogenic differentiated human mesenchymal stromal/stem cells.- In vitro expansion of human adipose-derived stem cells (ASCs).- siRNA silencing of adipogenic gene in ASCs.- Freezing and thawing of Adipose-derived stromal/stem cells (ASCs).- Isolation and Characterization of Mouse Adipose-Derived Stromal/Stem Cells.- Isolation of adipose-derived mesenchymal stromal/stem cells.- Isolation and purification of adipocyte-derived extracellular vesicles (AdEVs) using size-exclusion chromatography.- Differentiation of Adipose derived stromal/stem cells into Osteocytes.- Differentiation of mesenchymal stromal/stem cells from induced pluripotent stem cells.- Isolation and Characterization of Equine Mezenchymal Stromal/Stem Cell.