Immunohistochemistry and Immunocytochemistry

Simon Renshaw is the editor of Immunohistochemistry and Immunocytochemistry: Essential Methods, 2nd Edition, published by Wiley.

• List of Contributors vii Preface ixAcknowledgements xiChapter 1: Antibodies for Immunochemistry 1Mark Cooper and Sheriden LummasIntroduction 1Immunogens for Antibody Production 5Antibody Production 12Antibody Purification 16Fragment Antibody Preparations 20Antibody Labelling 21Antibody Stability and Storage 23References 24Chapter 2: The Selection of Reporter Labels 25Judith LangenickIntroduction 25Enzymatic Labels 26Fluorescence Detection 29References 32Chapter 3: Immunohistochemistry and Immunocytochemistry 35Simon RenshawSpecimen Formats for Immunochemistry 36Fixation 37Processing Tissue Blocks to Paraffin Wax 46Microtomy 47Tissue Microarrays 47Specimen Storage 48Decalcification 49Antigen Retrieval 50Controls 56Immunochemical Staining Techniques (Optimizing a New Antibody) 57Counterstains 71Mounting 74Troubleshooting 76Examples of Immunostaining Photomicrographs 76Acknowledgements 101References 101Chapter 4: Multiple Immunochemical Staining Techniques 103Sofia KochIntroduction 103Methods and Approaches 115References 122Chapter 5: Quality Assurance in Immunochemistry 123Peter Jackson and Michael GandyIntroduction 123Methods and Approaches 125Automated Immunochemical Staining 147Troubleshooting 149References 154Chapter 6: Automated Immunochemistry 157Emanuel Schenck and Simon RenshawIntroduction 157Methods and Approaches 160Other Forms of Automation 164References 168Chapter 7: Confocal Microscopy 169Ann WheelerIntroduction 169When Should Confocal be Used? 173Applications: For Example Co-localization, Quantification, 3D Visualization and Kinetics 173How To Set Up a Confocal Experiment? 174References 198Further Readings 198Chapter 8: Ultrastructural Immunochemistry 199Jeremy Skepper and Janet PowellIntroduction 199Methods and Approaches 207References 222Index 227