Cell Imaging Techniques

From the book reviews:“The different techniques are described in deep details, the chapters having an abstract, introduction, materials, methods, and, last but not least, notes; each method is described step by step with very, very useful links to the notes. … should be read not only by very specialized scientists, but also by those who usually work with classic microscopic methods, to open their minds and increase their appetite for more precise and complex measurements done on different level of complex biological structures.” (Ioan I. Ardelean, Bulletin of Micro and Nanoelectrotechnologies, Vol. 5 (3-4), December, 2014)“The goal is to provide seasoned microscopists with a list of protocols to add sophistication to their existing microscopy regimen. The intended audience is experienced researchers who are familiar with imaging in a core microscopy facility. … this book is more useful to microscopists who routinely use microscopic techniques in their research and are knowledgeable about the recent advances in technology. … an ideal reference for a core microscope facility. Each chapter is written independently by a different group of researchers and can stand alone.” (Latha Malaiyandi, Doody’s Book Reviews, December, 2013)

• Digital Images are Data – and Should be Treated as Such.- Epi-fluorescence Microscopy.-Live-Cell Migration and Adhesion Turnover Assays.-Multifluorescence Confocal Microscopy: Application for a Quantitative Analysis of HemostaticProteins in Human Venous Valve.-Colocalization Analysis in Fluorescence Microscopy.-A Time-Lapse Imaging Assay to Study Nuclear Envelope Breakdown.-Light Sheet Microscopy in Cell Biology.-Image-based High-throughput Screening for Inhibitors of Angiogenesis.-Intravital Microscopy to Image Membrane Trafficking in Live Rats.-Imaging Non-fluorescent Nanoparticles in Living Cells with Wavelength-Dependent.-Laser Scanning Cytometry. Principles and Applications: An Update.-Laser Capture Microdissection for Protein and NanoString RNA Analysis.-Viewing Dynamic Interactions of Proteins and a Model Lipid Membrane with Atomic Force Microscopy.-Mica Functionalization for Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy.-Measuring the Elastic Properties of Living Cells with Atomic Force Microscopy Indentation.-Colocalization Analysis in Fluorescence Microscopy.-A Time-Lapse Imaging Assay to Study Nuclear Envelope Breakdown.-Light Sheet Microscopy in Cell Biology.-Image-based High-throughput Screening for Inhibitors of Angiogenesis.-Intravital Microscopy to Image Membrane Trafficking in Live Rats.-Imaging Non-fluorescent Nanoparticles in Living Cells with Wavelength-Dependent.-Laser Scanning Cytometry. Principles and Applications: An Update.-Laser Capture Microdissection for Protein and NanoString RNA Analysis.-Viewing Dynamic Interactions of Proteins and a Model Lipid Membrane with Atomic Force Microscopy.-Mica Functionalization for Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy.-Measuring the Elastic Properties of Living Cells with Atomic Force Microscopy Indentation.-Atomic Force Microscopy Functional Imaging on Vascular Endothelial Cells.-Porosome: The Secretory NanoMachine in Cells.-Stereology andMorphometry of Lung Tissue.-A Novel Combined Imaging/Stereological Method for the Analysis of Human Sural Nerve Biopsies for Clinical Diagnosis.-Correlative Light–Electron Microscopy as a Tool to Study In vivo Dynamics and uUtrastructure of Intracellular Structures.-Photooxidation Technology for Correlative Light and Electron Microscopy.-Electron Microscopy of Endocytic Pathways.-Morphological Analysis of Autophagy.-Cytochemical Detection of Peroxisomes and Mitochondria.-Histochemical Detection of Lipid Droplets in Cultured Cells.-Environmental Scanning Electron Microscopy in Cell Biology.-Environmental Scanning Electron Microscopy Gold Immunolabeling in Cell Biology.-High Pressure Freezing for Scanning Transmission Electron Tomography Analysis of Cellular Organelles.-MALDI Imaging Mass Spectrometry for Direct Tissue Analysis.