Breast Cancer

Methods and Protocols

AvJian Cao

Häftad, Engelska, 2018

Del i serien Methods in Molecular Biology

1 202 kr

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Beskrivning

methods used in both clinical and research laboratories for testing genetic alterations (Genetic Detection of Breast Cancer) and (Isolation of Breast Cancer Cells); and methods used for mimicking human breast cancer in a living organism (In Vivo Experimental Models for Breast Cancer).

Produktinformation

Utgivningsdatum:2018-04-01
Mått:178 x 254 x 18 mm
Vikt:726 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:332
Förlag:Humana Press Inc.
ISBN:9781493980529

Utforska kategorier

Onkologi inom Medicin

Mer om författaren

JIAN CAORoom 004, Life Sciences BuildingDivision of Cancer PreventionDepartment of MedicineSchool of Medicine,Stony Brook UniversityStony Brook, NY 11794-5200631-632-1815email: Jian.Cao@stonybrookmedicine.edu

Innehållsförteckning

Basic Histopathological Methods and Breast Lesion Types for Research.- Clinical Applications for Immunohistochemistry of Breast Lesions.- Immunohistochemistry for Triple-Negative Breast Cancer.- In Situ Hybridization of Breast Cancer Markers.- Evaluation of Human Epidermal Growth Factor Receptor 2 (HER2) Gene Status in Human Breast Cancer Formalin-Fixed Paraffin Embedded (FFPE) Tissue Specimens by Fluorescence In Situ Hybridization (FISH).- Quantification of mRNA Levels Using Real-Time Polymerase Chain Reaction (PCR).- Detection of MiRNA in Cultured Cells or Xenograft Tissues of Breast Cancer.- Pyrosequencing Analysis for Breast Cancer DNA Methylome.- Vita-Assay ™ Method of Enrichment and Identification of Circulating Cancer Cells/Circulating Tumor Cells (CTCs).- Breast Cancer Stem Cell Isolation.- Cellular Apoptosis Assay of Breast Cancer.- Assessment of Matrix Metalloproteinases by Gel Zymography.- Assessment of Synthetic Matrix Metalloproteinase Inhibitors by Fluorogenic Substrate Assay.- Determination of Breast Cancer Cell Migratory Ability.- A Novel Collagen Dot Assay for Monitoring Cancer Cell Migration.- Three-Dimensional Assay for Studying Breast Cancer Cell Invasion.- A Combined Phagocytosis and Fluorescent Substrate Degradation Assay to Simultaneously Assess Cell Migration and Substrate Degradation.- Analysis of Invadopodia Formation in Breast Cancer Cells.- Patient-Derived Tumor Xenograft Models of Breast Cancer.- Monitoring Phosphatidic Acid Signaling in Breast Cancer Cells Using Genetically Encoded Biosensors.- 3D In Vitro Model for Breast Cancer Research Using Magnetic Levitation and Bioprinting Method.- Methods for Analyzing Tumor Angiogenesis in the Chick Chorioallantoic Membrane Model.- Pharmacokinetics and Pharmacodynamics in Breast Cancer Animal Models.- Intracellular Delivery of Fluorescently Labeled Polysaccharide Nanoparticles to Cultured Breast Cancer Cells.- Imaging Matrix Metalloproteinase Activity Implicated in Breast Cancer Progression.