Lymphoma

Methods and Protocols

AvRalf Küppers

Inbunden, Engelska, 2019

Del i serien Methods in Molecular Biology

1 858 kr

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Beskrivning

The new and updated chapters discuss the latest approaches for studying single cell RNA sequencing, whole genome sequencing data, CRISPR/Cas9-mediated gene knockout, protein interactions by a proximity ligation assay, and detection of circulating cell-free tumor DNA in lymphomas.

Produktinformation

Utgivningsdatum:2019-02-19
Mått:178 x 254 x 30 mm
Vikt:1 041 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:439
Upplaga:2
Förlag:Humana Press Inc.
ISBN:9781493991501

Utforska kategorier

Onkologi inom Medicin

Innehållsförteckning

Origin and Pathogenesis of B Cell Lymphomas.- Flow Cytometry for Non-Hodgkin and Hodgkin Lymphoma.- Laser-Based Microdissection of Single Cells from Tissue Sections and PCR Analysis of Rearranged Immunoglobulin Genes from Isolated Normal and Malignant Human B Cells.- PCR GeneScan and Heteroduplex Analysis of Rearranged Immunoglobulin or T-Cell Receptor Genes for Clonality Diagnostics in Suspect Lymphoproliferations.- Expression Cloning of Antibodies from Single Human B Cells.- Studying the Replication History of Human B Lymphocytes by Real-Time Quantitative (RQ)-PCR.- Stereotyped B Cell Immunoglobulins in B Cell Lymphomas.- Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies.- MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR.- Generation of Whole Genome Bisulfite Sequencing Libraries from Very Low DNA Input.- FISH and FICTION in Lymphoma Research.- NGS-Based High-Throughput Screen to Identify MicroRNAs Regulating Growth of B-Cell Lymphoma.- RNA Sequencing in B-Cell Lymphomas.- Studying Cancer Heterogeneity by Single Cell RNA-Sequencing.- Evaluation of Whole Genome Sequencing Data.- Protocols for CRISPR-Cas9 Screening in Lymphoma Cell Lines.- Lymphoma and Leukemia Cell Vulnerabilities and Resistance Identified by Compound Library Screens.- Proximity Ligation Assay.- Phospoproteomic Analysis of Signaling Pathways in Lymphomas.- Ultrasensitive Detection of Circulating Tumour DNA in Lymphoma via Targeted Hybridization Capture and Deep Sequencing of Barcoded Libraries.