Jean-Philippe Mocho (Edited By) J-P graduated in France as a Doctor in Veterinary Medicine at the start of the century. He practiced as a large animal vet across the French countryside, Wales, Scotland, and Northern Ireland. In 2009, he joined the Royal Veterinary College to dedicate his career to laboratory animals as a Named Veterinary Surgeon, providing his services to London's major academia and research institutes. His approach based on his experience of population medicine led him to the new shores of aquatic facilities. He devoted himself to the development of better care for zebrafish, thanks to experimental data supporting the 3Rs and the publication of recommendations. The latter was helped by J-P's leadership in activities of the Federation of European Laboratory Animal Science Associations, such as organising international congresses, chairing scientific committees, and convening working groups on aquatics.

• Part I: Part I 1: The zebrafish model: an introduction 2: Environmental enrichment for zebrafish 3: Dietary requirements for zebrafish 4: Non-infectious diseases of zebrafish 5: Infectious diseases of zebrafish 6: Adult zebrafish as a vertebrate model of ageing 7: Organization of the zebrafish facility: biosecurity, water quality, health monitoring and environmental impact Part II: Part II 8: The 3Rs in zebrafish research 9: Statistical considerations for the breeding of zebrafish and their use in experiments 10: Genotyping methods in zebrafish 11: Imaging of zebrafish 12: Behaviour in zebrafish 13: Anaesthesia, analgesia and euthanasia of zebrafish: protocol evaluation 14: Severity assignment for EU and UK procedures on zebrafish

Jean-Philippe Mocho (Edited By) J-P graduated in France as a Doctor in Veterinary Medicine at the start of the century. He practiced as a large animal vet across the French countryside, Wales, Scotland, and Northern Ireland. In 2009, he joined the Royal Veterinary College to dedicate his career to laboratory animals as a Named Veterinary Surgeon, providing his services to London's major academia and research institutes. His approach based on his experience of population medicine led him to the new shores of aquatic facilities. He devoted himself to the development of better care for zebrafish, thanks to experimental data supporting the 3Rs and the publication of recommendations. The latter was helped by J-P's leadership in activities of the Federation of European Laboratory Animal Science Associations, such as organising international congresses, chairing scientific committees, and convening working groups on aquatics.

• Part I: Part I 1: The zebrafish model: an introduction 2: Environmental enrichment for zebrafish 3: Dietary requirements for zebrafish 4: Non-infectious diseases of zebrafish 5: Infectious diseases of zebrafish 6: Adult zebrafish as a vertebrate model of ageing 7: Organization of the zebrafish facility: biosecurity, water quality, health monitoring and environmental impact Part II: Part II 8: The 3Rs in zebrafish research 9: Statistical considerations for the breeding of zebrafish and their use in experiments 10: Genotyping methods in zebrafish 11: Imaging of zebrafish 12: Behaviour in zebrafish 13: Anaesthesia, analgesia and euthanasia of zebrafish: protocol evaluation 14: Severity assignment for EU and UK procedures on zebrafish

Jean-Philippe Mocho (Edited By) J-P graduated in France as a Doctor in Veterinary Medicine at the start of the century. He practiced as a large animal vet across the French countryside, Wales, Scotland, and Northern Ireland. In 2009, he joined the Royal Veterinary College to dedicate his career to laboratory animals as a Named Veterinary Surgeon, providing his services to London's major academia and research institutes. His approach based on his experience of population medicine led him to the new shores of aquatic facilities. He devoted himself to the development of better care for zebrafish, thanks to experimental data supporting the 3Rs and the publication of recommendations. The latter was helped by J-P's leadership in activities of the Federation of European Laboratory Animal Science Associations, such as organising international congresses, chairing scientific committees, and convening working groups on aquatics.

• Part I: Part I 1: The zebrafish model: an introduction 2: Environmental enrichment for zebrafish 3: Dietary requirements for zebrafish 4: Non-infectious diseases of zebrafish 5: Infectious diseases of zebrafish 6: Adult zebrafish as a vertebrate model of ageing 7: Organization of the zebrafish facility: biosecurity, water quality, health monitoring and environmental impact Part II: Part II 8: The 3Rs in zebrafish research 9: Statistical considerations for the breeding of zebrafish and their use in experiments 10: Genotyping methods in zebrafish 11: Imaging of zebrafish 12: Behaviour in zebrafish 13: Anaesthesia, analgesia and euthanasia of zebrafish: protocol evaluation 14: Severity assignment for EU and UK procedures on zebrafish