This book constitutes the refereed proceedings of the 25th International Conference on Applications of Natural Language to Information Systems, NLDB 2020, held in Saarbrücken, Germany, in June 2020.*The 15 full papers and 10 short papers were carefully reviewed and selected from 68 submissions. The papers are organized in the following topical sections: semantic analysis; question answering and answer generation; classification; sentiment analysis; personality, affect and emotion; retrieval, conversational agents and multimodal analysis.*The conference was held virtually due to the COVID-19 pandemic.