This open access book constitutes the proceedings of the 29th International Conference on Tools and Algorithms for the Construction and Analysis of Systems, TACAS 2023, which was held as part of the European Joint Conferences on Theory and Practice of Software, ETAPS 2023, during April 22-27, 2023, in Paris, France.The 56 full papers and 6 short tool demonstration papers presented in this volume were carefully reviewed and selected from 169 submissions. The proceedings also contain 1 invited talk in full paper length, 13 tool papers of the affiliated competition SV-Comp and 1 paper consisting of the competition report.TACAS is a forum for researchers, developers, and users interested in rigorously based tools and algorithms for the construction and analysis of systems. The conference aims to bridge the gaps between different communities with this common interest and to support them in their quest to improve the utility, reliability, flexibility, and efficiency of tools and algorithms for building computer-controlled systems.