Igor L. Medintz is the U.S. Navy's Senior Scientist for Biosensors and Biomaterials at the U.S. Naval Research Laboratory in Washington, D.C. He obtained his Ph.D. from the City University of New York and then spent three years as a postdoctoral researcher at the University of California, Berkeley. He has coauthored over 200 scientific publications, 18 patents, and won several awards including the 2013 Arthur S. Flemming Award for Basic Science. W. Russ Algar is an Assistant Professor at the University of British Columbia in Vancouver, Canada, and a Canada Research Chair (Tier 2) and Michael Smith Foundation for Health Research Scholar. He obtained his Ph.D. from the University of Toronto, and then spent two years as a postdoctoral researcher at the U.S. Naval Research Laboratory in Washington, D.C., USA. He has authored over 70 scientific publications and won several awards and fellowships for his research. Philip Dawson obtained his Ph.D. under the guidance of Prof. Kent. After two years postdoctoral research at Caltech under Prof. H. Gray he became Associate Professor at the Scripps Research Institute in La Jolla, USA. His research field is synthetic protein chemistry. He has published more than 100 papers in international journals and has won several awards (Max Bergmann medal, Vincent du Vigneaud Award of the American Peptide Society).

• Contents to Volume 1List of Contributors xiPreface xviiPart I Chemistries 11 A Brief Introduction to Traditional Bioconjugate Chemisrty 3W. Russ Algar2 (3+2)-Doplar Cycloadditions in Bioconjugation 37Jason E. Hein3 Diels-Adler and Inverse Diels-Adler Reactions 67Roberto J. Brea and Neal K. Devaraj4 The Straudinger Ligation 97Olaia Nieto-Garcia, Marcie B. Jaffee, Michaela Muhlberg, and Christian P.R. Hackenberger5 Thiol-Ene Chemistry 117Neil B. Cramer and Christopher N. Bowman6 Ligand-Directed Tosyl and Acyl Imidazole Chemisrty 147Kazuya Matsuo and Itaru Hamachi7 Bioorthogonal Labeling of Cellular Proteins by Enzymatic and Related Mechanisms 165Scott A. Walper, Kendrick B. Turner, and Igor L. Medintz8 Metal-Mediated Bioconjugation 231Justin M. ChalkerContents to Volume 2List of Contributors xiiiPreface xixPart II applications 2719 Protein and Anitbody Labeling 273Angela M. Mariani and Kim E. Sapsford10 Activity-Based Protein Profiling 305Elaine V. Wolf and Steven H.L. Verhelst11 Nucleic Acid Labeling, Ligation and Modicfication 335Afah H. El-Sagheer and Tom Brown12 Chemoselective Reactions for Glycan Labeling 363Janet E. McCombs and Jennifer J. Kholer13 Chemoselective Attachment of Lipids to Proteins 391Christian F. W. Becker14 In Vivo Applications of Bioorthogonal Chemistries 417Chelsea G. Gordon and Carolyn R. Bertozzi15 Immobilization of Biomolecular Probes for Arrays and Assays: Critical Aspects of Biointerfaces 459Stella H. North and Chris Rowe Taitt16 Chemical Ligations in the Design of Hydrogel Materials 497Scott H. Medina and Joel P. Schneider17 Nanoparticle Bioconjugates: Materials that Benefit from Chemoselective and Bioorthogonal Ligation Chemistires 543Melissa Massey and W. Russ Algar18 Applications of Engineered Viral Nanoparticles in Materials and Medicine 631Michael D. Glidden II, John F. Edelbrock, Amy M. Wen, Sourabh Shukla, Yingfang Ma, Roger H. French, Jonathan K. Pokorski, and Nicole F. SteinmetzIndex 711