Usha MD Professor and Head, Department of Pathology; Incharge, UGC Advanced Immunodiagnostic Training & Research Centre, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, IndiaGyanendra Kumar Sonkar PhD Assistant Professor, Department of Biochemistry, King George’s Medical University, Lucknow, Uttar Pradesh, IndiaSangeeta Singh PhD Ex-Senior Research Fellow, UGC Advanced Immunodiagnostic Training and Research Centre, Division of Immunopathology, Department of Pathology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India

• IntroductionTests for cell mediated immunity (CMI)Lymphocytes typing by Flow CytometryDiagnosis of Kala-Azar by PCR techniqueTest for myeloma profile and qualitative assessment of serum proteinsAgarose gel electrophoresis for qualitative assessment of serum proteinsCellulose Acetate ElectrophoresisAgar Gel Immunoelectrophoresis for typing of myelomaMicro Agar Gel Double Diffusion TechniqueCounter Current ImmunoelectrophoresisSingle Radial Immunodiffusion and Immunoturbidometric methods for the estimation of ImmunoglobulinsEstimation of complement C3, C4 and hemolytic assay for serum complementDemonstration of immune complexes and complement by indirect immuno peroxidase methodTest for autoimmune diseasesTest for Autoantibodies by ELISA techniqueAcid Fuchsin Orange G in kidney biopsy for demonstration of immune complexesDetection of tumor markers in paraffin embedded sections by immunohistochemistryDetection of circulating immune complexes (CIC) in the serumDemonstration of immune complexes, antibodies and complements in renal biopsy by immunofluorescenceHLA typing and Cross Match test in solid organ transplantationHLA B27 typing by PCR methodFluorescence in-situ hybridisation: methods and applicationsDetection of cytokines (IL-6, IL-8, TNF-a)Western Blot or Tests for detection of antibody to extractable nuclear antigenDetection of anti-thyroglobulin antibodies by hemagglutination methodDistillation and demineralization of waterRaising of polyclonal anti human anti-IgG in rabbit

Usha MD Professor and Head, Department of Pathology; Incharge, UGC Advanced Immunodiagnostic Training & Research Centre, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, IndiaGyanendra Kumar Sonkar PhD Assistant Professor, Department of Biochemistry, King George’s Medical University, Lucknow, Uttar Pradesh, IndiaSangeeta Singh PhD Ex-Senior Research Fellow, UGC Advanced Immunodiagnostic Training and Research Centre, Division of Immunopathology, Department of Pathology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India

• IntroductionTests for cell mediated immunity (CMI)Lymphocytes typing by Flow CytometryDiagnosis of Kala-Azar by PCR techniqueTest for myeloma profile and qualitative assessment of serum proteinsAgarose gel electrophoresis for qualitative assessment of serum proteinsCellulose Acetate ElectrophoresisAgar Gel Immunoelectrophoresis for typing of myelomaMicro Agar Gel Double Diffusion TechniqueCounter Current ImmunoelectrophoresisSingle Radial Immunodiffusion and Immunoturbidometric methods for the estimation of ImmunoglobulinsEstimation of complement C3, C4 and hemolytic assay for serum complementDemonstration of immune complexes and complement by indirect immuno peroxidase methodTest for autoimmune diseasesTest for Autoantibodies by ELISA techniqueAcid Fuchsin Orange G in kidney biopsy for demonstration of immune complexesDetection of tumor markers in paraffin embedded sections by immunohistochemistryDetection of circulating immune complexes (CIC) in the serumDemonstration of immune complexes, antibodies and complements in renal biopsy by immunofluorescenceHLA typing and Cross Match test in solid organ transplantationHLA B27 typing by PCR methodFluorescence in-situ hybridisation: methods and applicationsDetection of cytokines (IL-6, IL-8, TNF-a)Western Blot or Tests for detection of antibody to extractable nuclear antigenDetection of anti-thyroglobulin antibodies by hemagglutination methodDistillation and demineralization of waterRaising of polyclonal anti human anti-IgG in rabbit

Usha MD Professor and Head, Department of Pathology; Incharge, UGC Advanced Immunodiagnostic Training & Research Centre, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, IndiaGyanendra Kumar Sonkar PhD Assistant Professor, Department of Biochemistry, King George’s Medical University, Lucknow, Uttar Pradesh, IndiaSangeeta Singh PhD Ex-Senior Research Fellow, UGC Advanced Immunodiagnostic Training and Research Centre, Division of Immunopathology, Department of Pathology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India

• IntroductionTests for cell mediated immunity (CMI)Lymphocytes typing by Flow CytometryDiagnosis of Kala-Azar by PCR techniqueTest for myeloma profile and qualitative assessment of serum proteinsAgarose gel electrophoresis for qualitative assessment of serum proteinsCellulose Acetate ElectrophoresisAgar Gel Immunoelectrophoresis for typing of myelomaMicro Agar Gel Double Diffusion TechniqueCounter Current ImmunoelectrophoresisSingle Radial Immunodiffusion and Immunoturbidometric methods for the estimation of ImmunoglobulinsEstimation of complement C3, C4 and hemolytic assay for serum complementDemonstration of immune complexes and complement by indirect immuno peroxidase methodTest for autoimmune diseasesTest for Autoantibodies by ELISA techniqueAcid Fuchsin Orange G in kidney biopsy for demonstration of immune complexesDetection of tumor markers in paraffin embedded sections by immunohistochemistryDetection of circulating immune complexes (CIC) in the serumDemonstration of immune complexes, antibodies and complements in renal biopsy by immunofluorescenceHLA typing and Cross Match test in solid organ transplantationHLA B27 typing by PCR methodFluorescence in-situ hybridisation: methods and applicationsDetection of cytokines (IL-6, IL-8, TNF-a)Western Blot or Tests for detection of antibody to extractable nuclear antigenDetection of anti-thyroglobulin antibodies by hemagglutination methodDistillation and demineralization of waterRaising of polyclonal anti human anti-IgG in rabbit