Diagnostic Flow Cytometry in Cytology

Pranab Dey is Professor in Department of Cytology and Gynecologic Pathology at Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh. Professor Dey completed M.D. (pathology) from PGIMER, Chandigarh and FRCPath (cytopathology) from Royal College of Pathologist, London. He has been a Consultant of cytology and gynecologic pathology in PGIMER for last 29 years and Professor since last 11 years. Professor Dey has keen interest in flow cytometry and artificial intelligence and conducted many research projects and has pioneered works on DNA flow cytometry, image morphometry, mono-layered cytology and cytomorphologic findings of various lesions on cytology smears. He is a well published author, has published several books in the field of cytology and gynecologic pathology, 300 publications in international journals; 150 original research works and 30 review articles). Professor Dey is a member of various societies.

• Part I: Technical aspects.- Introduction and history of flow cytometry.- Basic principles of flow cytometry and instrumentation.- Specimen preparation and data acquisition.- Interpretation of graphs and quality control.- Fluorescent probes and different useful markers for flow cytometry.- Nuclei acid dye for flow cytometry.- Part II: Applications of flow cytometry.- Application of flow cytometry in lymphoid neoplasms.- Application of flow cytometry in body cavity fluids.- Application of flow cytometry in solid tumors.- Cell cycle analysis, DNA flow cytometry and ploidy analysis.- Future applications of flow cytometry.- Practical cases in flow cytometry.