This book constitutes the proceedings of the 7th International Conference on Machine Learning for Cyber Security, ML4CS 2025, which taking place during December 12-14, 2025 held in Hangzhou, China.The 18 full papers presented in this book were carefully reviewed and selected from 97 submissions. ML4CS is a well-recognized annual international forum for AI-driven security researchers to exchange ideas and present their works. The conference focus on topics such as blockchain, network security, system security, software security, threat intelligence, cybersecurity situational awareness and much many more.