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While the theoretical basis of centrifugal separation remains the same, recent years have seen dramatic changes in both the design of centrifuges and the range of people that use them. This study is designed to reflect these changes by providing detailed protocols covering all types of separation, from DNA to cells. Guidelines are also given to help researchers devise new types of separation protocols. The book includes program source codes for calculation and simulation programs that are beneficial for quantitative centrifugation methods.
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As a modern composite scientific discipline, Cell Biology has expanded and moved forward rapidly in recent years. Cell Biologists now require a wide range of techniques, including those of analytical biochemistry and microscopy in all its diverse forms. These are often used alongside the techniques of molecular biology and molecular genetics. This book contains numerous useful protocols, covering light and electron microscopy, cell culture, cell separation, subcellular fractionation, organelle and membrane isolation, and the use of in vitro reassembly systems in Cell Biology. Many of these protocols feature helpful notes and safety information for practical application. The format favours easy use at the bench with space for notes and important safety information. An appendix contains essential analytical information that will prove invaluable to those working on all aspects of cell biology. This book will be of interest to students and more experienced cell biologists, as well as molecular biologists and those working in genomics and proteomics who are looking for cellular techniques to validate their findings within intact cells.