Professor Elmar Heinzle, Universität des Saarlandes, Germany Dr. Charles Cooney, MIT, USABoth internationally-recognised experts in biochemical engineering and modelingDr Arno Biwer, MIT/ Universität des Saarlandes, Germany

• Preface. Acknowledgements.List of Contributors.Part I: Theoretical Introduction.1. Introduction.1.1. Bioprocesses.1.2. Modeling and Assessment in Process Development.2. Development of Bioprocesses.2.1. Types of Bioprocesses and Bioproducts.2.2. Bioreaction Stoichiometry, Thermodynamics and Kinetics.2.3. Elements of Bioprocesses (Unit Operations and Unit Processes).2.4. The Development Process.3. Modeling and Simulation of Bioprocesses.3.1. Problem Structuring, Process Analysis and Process Scheme.3.2. Implementation and Simulation.3.3. Uncertainty Analysis.4. Sustainability Assessment.4.1. Sustainability.4.2. Economic Assessment.4.3. Environmental Assessment.4.4. Assessing Social Aspects.4.5. Interactions Between the Sustainability Dimensions.Part II: Bioprocess Case Studies.Introduction to Case Studies 5. Citric Acid - Alternative Process Using Starch.5.1. Introduction.5.2. Fermentation Model.5.3. Process Model.5.4. Inventory Analysis.5.5. Environmental Assessment.5.6. Economic Assessment.5.7. Conclusions.6. Pyruvic Acid - Fermentation with Alternative Downstream Processes.6.1. Introduction.6.2. Fermentation Model.6.3. Process Model.6.4. Inventory Analysis.6.5. Environmental Assessment.6.6. Economic Assessment.6.7. Conclusions.7. L-Lysine - Coupling of Bioreaction and Process Model.7.1. Introduction.7.2. Basic Strategy.7.3. Bioreaction Model.7.4. Process Model.7.5. Coupling of Bioreaction and Process Model.7.6. Results and Discussion.8. Riboflavin – Vitamin B2.8.1. Introduction.8.2. Biosynthesis and Fermentation.8.3. Production Process and Process Model.8.4. Inventory Analysis.8.5 Ecological Assessment.8.6 Economic Assessment.8.7 Discussion and Concluding Remarks.9. α-Cyclodextrin.9.1. Introduction.9.2. Reaction Model.9.3. Process Model.9.4. Inventory Analysis.9.5. Environmental Assessment.9.6. Economic Assessment.9.7. Conclusions.10. Penicillin V.10.1. Introduction.10.2. Modeling Base Case.10.3. Inventory Analysis.10.4. Environmental Assessment.10.5. Economic Assessment.10.6. Monte Carlo Simulations.10.7. Conclusions.11. Recombinant Human Serum Albumin.11.1. Introduction.11.2. Bioreaction Model.11.3. Process Model.11.4. Economic Assessment.11.5. Ecological Assessment.11.6. Conclusions.12. Recombinant Human Insulin.12.1. Introduction.12.2. Market Analysis and Design Basis.12.3. Economic Assessment.12.4. Throughput Increase Options.12.5. Conclusions.13. Monoclonal Antibodies.13.1. Introduction.13.2. Process Model.13.3. Inventory Analysis.13.4. Economic Assessment.13.5. Environmental Assessment.13.6. Uncertainty Analysis.13.6. Conclusions.14. α-1-Antitrypsin from Transgenic Plant Cell Suspension Cultures.14.1. Introduction.14.2. Process Description.14.3. Model Description.14.4. Discussion.14.5 Conclusions.15. Plasmid DNA.15.1. Introduction.15.2. Model Description.15.3. Inventory Analysis.15.4. Economic Assessment.15.5. Environmental Assessment.15.6. Discussion.15.7. Conclusions.Index.

Professor Elmar Heinzle, Universität des Saarlandes, Germany Dr. Charles Cooney, MIT, USABoth internationally-recognised experts in biochemical engineering and modelingDr Arno Biwer, MIT/ Universität des Saarlandes, Germany

• Preface. Acknowledgements.List of Contributors.Part I: Theoretical Introduction.1. Introduction.1.1. Bioprocesses.1.2. Modeling and Assessment in Process Development.2. Development of Bioprocesses.2.1. Types of Bioprocesses and Bioproducts.2.2. Bioreaction Stoichiometry, Thermodynamics and Kinetics.2.3. Elements of Bioprocesses (Unit Operations and Unit Processes).2.4. The Development Process.3. Modeling and Simulation of Bioprocesses.3.1. Problem Structuring, Process Analysis and Process Scheme.3.2. Implementation and Simulation.3.3. Uncertainty Analysis.4. Sustainability Assessment.4.1. Sustainability.4.2. Economic Assessment.4.3. Environmental Assessment.4.4. Assessing Social Aspects.4.5. Interactions Between the Sustainability Dimensions.Part II: Bioprocess Case Studies.Introduction to Case Studies 5. Citric Acid - Alternative Process Using Starch.5.1. Introduction.5.2. Fermentation Model.5.3. Process Model.5.4. Inventory Analysis.5.5. Environmental Assessment.5.6. Economic Assessment.5.7. Conclusions.6. Pyruvic Acid - Fermentation with Alternative Downstream Processes.6.1. Introduction.6.2. Fermentation Model.6.3. Process Model.6.4. Inventory Analysis.6.5. Environmental Assessment.6.6. Economic Assessment.6.7. Conclusions.7. L-Lysine - Coupling of Bioreaction and Process Model.7.1. Introduction.7.2. Basic Strategy.7.3. Bioreaction Model.7.4. Process Model.7.5. Coupling of Bioreaction and Process Model.7.6. Results and Discussion.8. Riboflavin – Vitamin B2.8.1. Introduction.8.2. Biosynthesis and Fermentation.8.3. Production Process and Process Model.8.4. Inventory Analysis.8.5 Ecological Assessment.8.6 Economic Assessment.8.7 Discussion and Concluding Remarks.9. α-Cyclodextrin.9.1. Introduction.9.2. Reaction Model.9.3. Process Model.9.4. Inventory Analysis.9.5. Environmental Assessment.9.6. Economic Assessment.9.7. Conclusions.10. Penicillin V.10.1. Introduction.10.2. Modeling Base Case.10.3. Inventory Analysis.10.4. Environmental Assessment.10.5. Economic Assessment.10.6. Monte Carlo Simulations.10.7. Conclusions.11. Recombinant Human Serum Albumin.11.1. Introduction.11.2. Bioreaction Model.11.3. Process Model.11.4. Economic Assessment.11.5. Ecological Assessment.11.6. Conclusions.12. Recombinant Human Insulin.12.1. Introduction.12.2. Market Analysis and Design Basis.12.3. Economic Assessment.12.4. Throughput Increase Options.12.5. Conclusions.13. Monoclonal Antibodies.13.1. Introduction.13.2. Process Model.13.3. Inventory Analysis.13.4. Economic Assessment.13.5. Environmental Assessment.13.6. Uncertainty Analysis.13.6. Conclusions.14. α-1-Antitrypsin from Transgenic Plant Cell Suspension Cultures.14.1. Introduction.14.2. Process Description.14.3. Model Description.14.4. Discussion.14.5 Conclusions.15. Plasmid DNA.15.1. Introduction.15.2. Model Description.15.3. Inventory Analysis.15.4. Economic Assessment.15.5. Environmental Assessment.15.6. Discussion.15.7. Conclusions.Index.

Professor Elmar Heinzle, Universität des Saarlandes, Germany Dr. Charles Cooney, MIT, USABoth internationally-recognised experts in biochemical engineering and modelingDr Arno Biwer, MIT/ Universität des Saarlandes, Germany

• Preface. Acknowledgements.List of Contributors.Part I: Theoretical Introduction.1. Introduction.1.1. Bioprocesses.1.2. Modeling and Assessment in Process Development.2. Development of Bioprocesses.2.1. Types of Bioprocesses and Bioproducts.2.2. Bioreaction Stoichiometry, Thermodynamics and Kinetics.2.3. Elements of Bioprocesses (Unit Operations and Unit Processes).2.4. The Development Process.3. Modeling and Simulation of Bioprocesses.3.1. Problem Structuring, Process Analysis and Process Scheme.3.2. Implementation and Simulation.3.3. Uncertainty Analysis.4. Sustainability Assessment.4.1. Sustainability.4.2. Economic Assessment.4.3. Environmental Assessment.4.4. Assessing Social Aspects.4.5. Interactions Between the Sustainability Dimensions.Part II: Bioprocess Case Studies.Introduction to Case Studies 5. Citric Acid - Alternative Process Using Starch.5.1. Introduction.5.2. Fermentation Model.5.3. Process Model.5.4. Inventory Analysis.5.5. Environmental Assessment.5.6. Economic Assessment.5.7. Conclusions.6. Pyruvic Acid - Fermentation with Alternative Downstream Processes.6.1. Introduction.6.2. Fermentation Model.6.3. Process Model.6.4. Inventory Analysis.6.5. Environmental Assessment.6.6. Economic Assessment.6.7. Conclusions.7. L-Lysine - Coupling of Bioreaction and Process Model.7.1. Introduction.7.2. Basic Strategy.7.3. Bioreaction Model.7.4. Process Model.7.5. Coupling of Bioreaction and Process Model.7.6. Results and Discussion.8. Riboflavin – Vitamin B2.8.1. Introduction.8.2. Biosynthesis and Fermentation.8.3. Production Process and Process Model.8.4. Inventory Analysis.8.5 Ecological Assessment.8.6 Economic Assessment.8.7 Discussion and Concluding Remarks.9. α-Cyclodextrin.9.1. Introduction.9.2. Reaction Model.9.3. Process Model.9.4. Inventory Analysis.9.5. Environmental Assessment.9.6. Economic Assessment.9.7. Conclusions.10. Penicillin V.10.1. Introduction.10.2. Modeling Base Case.10.3. Inventory Analysis.10.4. Environmental Assessment.10.5. Economic Assessment.10.6. Monte Carlo Simulations.10.7. Conclusions.11. Recombinant Human Serum Albumin.11.1. Introduction.11.2. Bioreaction Model.11.3. Process Model.11.4. Economic Assessment.11.5. Ecological Assessment.11.6. Conclusions.12. Recombinant Human Insulin.12.1. Introduction.12.2. Market Analysis and Design Basis.12.3. Economic Assessment.12.4. Throughput Increase Options.12.5. Conclusions.13. Monoclonal Antibodies.13.1. Introduction.13.2. Process Model.13.3. Inventory Analysis.13.4. Economic Assessment.13.5. Environmental Assessment.13.6. Uncertainty Analysis.13.6. Conclusions.14. α-1-Antitrypsin from Transgenic Plant Cell Suspension Cultures.14.1. Introduction.14.2. Process Description.14.3. Model Description.14.4. Discussion.14.5 Conclusions.15. Plasmid DNA.15.1. Introduction.15.2. Model Description.15.3. Inventory Analysis.15.4. Economic Assessment.15.5. Environmental Assessment.15.6. Discussion.15.7. Conclusions.Index.