Tumor Necrosis Factor

Name: Tumor Necrosis Factor
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Methods and Protocols

AvAngelo Corti,Pietro Ghezzi

2014

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Beskrivning

A comprehensive collection of readily reproducible techniques for TNF production, characterization, mutagenesis, and detection in biological specimens. Also included are several in vitro assays and animal models for studying the role of TNF in various TNF-related diseases and in cancer. The collection offers molecular and cellular biologists, pharmacologists, and toxicologists a diverse set of versatile, cutting-edge tools for illuminating the pathophysiological roles of TNF in disease and for identifying new drugs.

Produktinformation

Utgivningsdatum:2014-05-14
Språk:Engelska
Förlag:Humana Press Inc.
EAN:9781592597710

Utforska kategorier

Onkologi inom Medicin

Innehållsförteckning

Table of Contents; Tumor Necrosis Factor: Methods and Protocols; Edited by Angelo Corti and Pietro Ghezzi; 1 Tumor Necrosis Factor as a Pharmacological Target Pietro Ghezzi and Antony Cerami; 2 Production and Characterization of Recombinant Human and Murine TNF Flavio Curnis and Angelo Corti; 3 Purification of TNF binding proteins Hartmut Engelmann, Dani Aderka and David Wallach; 4 Production and Characterization of Receptor Specific TNF Muteins Paul Ameloot and Peter Brouckaert; 5 TNF[alpha] Promoter Polymorphysms Silvia Fargion, Luca Valenti, Paola Dongiovanni, and Anna Ludovica Fracanzani; 6 Detection of TNF and TNF Receptor mRNA in Cells and Tissues Roberto Furlan, Giuliana Salani, Alessandra Bergami, and Gianvito Martino; 7 Immuno-Histochemical Localization of TNF[alpha] and its Receptors Paolo Bigini and Tiziana Mennini; 8 TNF Signaling: Key Protocols Marta Muzio and Simona Saccani; 9 More Than One Way to Die: Methods to Determine TNF-Induced Apoptosis and Necrosis Tom Vanden Berghe, Geertrui Denecker, Greet Brouckaert, Dmitri Krysko, Katharina Dherde, and Peter Vandenabeele; 10 Assessment of the Endothelial TNF[alpha]-Induced Damage Through the Loss of its Barrier Function Elisabetta Ferrero; 11 Genetic Engineering in the Mouse: Tuning TNF/TNF-R Expression Eleni Douni, Maria Alexiou, and George Kollias; 12 Animal Models of TNF[alpha]-Mediated Diseases of the Central Nervous System Roberto Furlan, Pia Villa, Giorgio Senaldi, and Gianvito Martino; 13 In Vivo Vascular Leakage Assay Maria Elena Ferrero; 14 Animal Models of Endotoxic Shock Pia Villa and Pietro Ghezzi; 15 Collagen-Induced Arthritis as a Model for Rheumatoid Arthritis Richard O. Williams; 16 Miocardial Infarction: Animal Models Stefano Chimenti, Eleonora Carlo, Serge Masson, Antonio Bai, and Roberto Latini; 17 Tumor Vascular Therapy with TNF: Critical Review on Animal Models Timo L. M. ten Hagen and Alexander M.M. Eggermont; 18 Strategies for Improving the Anti-Neoplastic Activity of TNF by Tumor Targeting Angelo Corti