Bioanalytical Reviews – serie

This book is dedicated to label-free, non-invasive monitoring of cell-based assays and it comprises the most widely applied techniques.

This book is dedicated to label-free, non-invasive monitoring of cell-based assays and it comprises the most widely applied techniques.

This book provides a selection of recent developments in scanning ion conductance microscopy (SICM) technology and applications.

This book provides a selection of recent developments in scanning ion conductance microscopy (SICM) technology and applications. In recent years, SICM has been applied in an ever-increasing number of areas in the bioanalytical sciences. SICM is based on an electrolyte-filled nanopipette with a nanometer-scale opening, over which an electric potential is applied. The induced ion current is measured, which allows to directly or indirectly quantify various physical quantities such as pipette-sample distance, ion concentration, sample elastic modulus among many others. This makes SICM well suited for applications in electrolytes - most prominently for the study of live cells.This book starts with a historic overview starting from the days of the invention of SICM by Paul Hansma at the University of California at Santa Barbara in 1989. SICM is a member of the family of scanning probe microscopies. It is related to another prominent member of the family, atomic force microscopy (AFM), which has found application in almost any field of nanoscale science. The advantages and disadvantages of SICM over AFM are also outlined. One of the most effective and break-through applications of SICM nanopipettes is in electrochemistry. The different routes and applications for doing electrochemistry using nanopipettes are also discussed. In addition the book highlights the ability of SICM for surface positioning with nanometer precision to open up new vistas in patch clamp measurements subcellular structures. Finally the book presents one research area where SICM has been making a lot of contributions, cardiac research and the endeavors to combine SICM with super-resolution optical microscopy for highest-resolution joint topography and functional imaging.

This volume highlights the potentials as well as the limits and challenges of human breath analysis and describes the current efforts made to advance this promising technology from bench to bed.

This volume highlights the potentials as well as the limits and challenges of human breath analysis and describes the current efforts made to advance this promising technology from bench to bed.

Expert authors provide critical, in-depth reviews of available methods for retrieving selective information out of complex biological systems. Coverage of this volume includes sensor development for the detection of small analytes, monitoring of biomolecular interactions, analysis of cellular function, development of diagnostic tools.

This book reviews several aspects of the biological response to nanoscale particles on a molecular and cellular level. Nanoscale materials and nanoscale particles in particular have interesting properties and beneficial applications. While they thus have entered our daily lifes on many different levels (from electronics, over textiles, packaging or surface modifications, to biomedical applications), general rules describing their interaction with biological structures and biological matter are still difficult to derive. The existing literature suggests a variety of interaction schemes between nanoparticles and biological objects, not dispelling the public concerns about possible health effects and harmful properties. A systematic approach to the problem is needed and timely.This book specifically emphasizes bioanalytical problems starting from the characterization of the nanomaterials to the pitfalls and potential artifacts of state-of-the-art cytotoxicityassays that are frequently used to study harmful effects on cells. It also highlights the application of label-free bioanalytical techniques that can potentially complement the present approaches and hence provide new perspectives on this highly discussed cutting-edge field of research and public concern.

Expert authors provide critical, in-depth reviews of available methods for retrieving selective information out of complex biological systems. Sensors, probes and devices are present and future tools of medicinal diagnostics, environmental monitoring, food analysis and molecular biology. These are based on fluorescence, electrochemistry and mass spectrometry. Coverage of this volume includes sensor development for the detection of small analytes, monitoring of biomolecular interactions, analysis of cellular function, development of diagnostic tools.

This volume offers a careful selection of trend-setting topics in the field. In-depth review articles illustrate current trends in the field. Experienced experts presenta comprehensive overview concerning the electrochemical biosensing of glucose for diabetes care from an industrial research and development perspectivea survey of bioassay applications for individually addressable electrochemical arrays, focusing on liquid-phase bioanalytical assaysa review of recent advances in the development of electronic tongues based on the use of biosensor arrays coupled with advanced chemometric data analysisnovel strategies of DNA biosensor development  and corresponding applications for studies of DNA damagea survey of recent trends in the electrochemistry of redox proteins, including the increasing diversity of redox proteins used in electrochemical studies, novel immobilization strategies, and biosensor / biofuel cell applicationsan overview of electrochemical sensing of blood gases with advanced sensor conceptsa survey of recent bioelectroanalytical studies with high spatial resolution using scanning electrochemical microscopy with a  wide range of applications covering imaging of living cells, studies of metabolic activity, imaging of local enzyme activity, and studies of transport through biolayersThis timely collection will be of interest not only for experts in the field, but also to students and their teachers in disciplines that include analytical chemistry, biology, electrochemistry, and various interdisciplinary research areas.

This book reviews several aspects of the biological response to nanoscale particles on a molecular and cellular level.