Receptor Purification - Böcker

Receptor Purification is a unique practical two-volume guide to the purification of most receptor types including receptors for neurotransmitters polypeptide hormones steroid hormones and a variety of other ligands involved in many biological processes. Today's methods of receptor purification have evolved from years of research on the mechanisms of receptor actions where they were needed to discover receptor properties and ultimately for use in cloning. Receptor Purification provides a comprehensive source of techniques to enable the purification of cloned receptors as well as a handy reference on purification of receptors from cells and tissues in a physiological context. Designed to give investigators the best possible selection of approaches to a purification problem alternative methods are provided where applicable. In addition Receptor Purification features chapters on specific agents-such as covalent binding ligands-that will greatly simplify some purification processes. Many of the leading laboratories whose work has led to the purification of a wide variety of receptors are represented in the contributions to these two volumes.In some chapters information has been added on the applications of purified receptors while others focus on the cloning of the receptors. The purification of receptors is group-based on functional ligands. The first volume is devoted to the purification of receptors that bind neurotransmitters and a wide variety of polypeptide hormones. Volume 2 covers the steroid receptors and associated proteins such as vitamin D-3 and retinoic acid receptors. Other polypeptide receptors such as those involved in

The purpose of these volumes is to provide a reference work for the methods of purifying many of the receptors we know about. This becomes increasingly important as full-length recep- tors are overexpressed in bacteria or in insect cell systems. A major problem for abundantly expressed proteins will be their purification. In addition to purification protocols, many other de- tails can be found concerning an individual receptor that may not be available in standard texts or monographs. No book of this type is available as a compendium of purification procedures. Receptor Purification provides protocols for the purification of a wide variety of receptors. These include receptors that bind: neurotransmitters, polypeptide hormones, steroid hormones, and ligands for related members of the steroid supergene family and others including receptors involved in bacterial motion. The text of this information is substantial so as to require its publica- tion in two volumes. Consequently, a division was made by grouping receptors depending upon the nature of their ligands.Thus, in volume 1 there are contributions on serotonin receptors, adrenergic receptors, the purification of GTP-binding proteins, opioid receptors, neurotensin receptor, luteinizing hormone re- ceptor, human chorionic gonadotropin receptor, follicle stimulat- ing hormone receptor, thyrotropin receptor, prolactin receptor, epidermal growth factor receptor, platelet derived growth factor receptor, colony stimulating factor receptor, insulinlike growth factor receptors, insulin receptor, fibronectin receptor, interferon receptor, and the cholecystokinin receptor.

The purpose of these volumes is to provide a reference work for the methods of purifying many of the receptors we know about. This be­ comes increasingly important as full-length receptors are overexpressed in bacteria or in insect cell systems. A major problem for abundantly expressed proteins will be their purification. In addition to purification protocols, many other details can be found concerning an individual receptor that may not be available in standard texts or monographs. No book of this type is available as a compendium of purification procedures. Receptor Purification provides protocols for the purification of a wide variety of receptors. These include receptors that bind: neurotransmit­ ters, polypeptide hormones, steroid hormones, and ligands for related members of the steroid supergene family and others, including receptors involved in bacterial motion. The text of this information is substantial, so as to require its publication in two volumes. Consequently, a division was made by grouping receptors by the nature of their ligands. Thus, in Volume One there are contributions on serotonin receptors, adrenergic receptors, the purification of GTP-binding proteins, opioid receptors, neurotensin receptor, luteinizing hormone receptor, human chorionic gonadotropin receptor, follicle stimulating hormone receptor, thyro­ tropin receptor, prolactin receptor, epidermal growth factor receptor, platelet derived growth factor receptor, colony stimulating factor recep­ tor, insulin-like growth factor receptors, insulin receptor, fibronectin receptor, interferon receptor, and the cholecystokinin receptor.