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      Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas

      Molecular Genetics; Lung and Breast Carcinomas

      AvM. A. Hayat

      Inbunden, Engelska, 2004

      2 509 kr

      Beställningsvara. Skickas inom 10-15 vardagar. Fri frakt över 249 kr.

      Beskrivning

      The various cell types have traditionally been recognized and classified according to their appearance in the light microscope following the process of fixing, processing, sectioning, and staining tissues that is known as histology. Classical histology has been augmented by immunohistochemistry (the use of specific antibodies to stain particular molecular species in situ). Immunohistochemistry has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system.

      Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step-by-step instructions on the methods of additional molecular technologies such as DNA microarrays, and microdissection, along with the benefits and limitations of each method. The topics of region-specific gene expression, its role in cancer development and the techniques that assist in the understanding of the molecular basis of disease are relevant and necessary in science today, ensuring a wide audience for this book.



      • The only book available that translates molecular genetics into cancer diagnosis
      • Provides the readers with tools necessary to perform and optimize sensitive, powerful techniques, including immunohistochemistry and fluorescence in situ hybridization, used in tumor diagnosis
      • Written by experts in this field, the book provides theoretical considerations as well as practical approaches to carry out effectively these techniques
      • Offers suggestions, tips, cautions, and guidelines to avoid artifacts and misdiagnosis
      • Introduces new techniques to detect genes and proteins involved in the initiation and progression of cancer
      • Covers the latest developments and a wide range of applications to the detection of antigens and single-copy DNA and RNA
      • Written in a uniform format, each chapter includes Introduction, Materials required, step-by-step detailed Methods, Results, Discussion, and comprehensive up-to-date References

      Produktinformation

      • Utgivningsdatum:2004-06-16
      • Mått:216 x 276 x 32 mm
      • Vikt:2 130 g
      • Format:Inbunden
      • Språk:Engelska
      • Antal sidor:556
      • Förlag:Elsevier Science
      • ISBN:9780123339416

      Utforska kategorier

      • Onkologi inom Medicin

      Mer om författaren

      Dr. Hayat has published extensively in the fields of microscopy, cytology, immunohistochemistry, immunocytochemistry, and antigen retrieval methods. He is Distinguished Professor, Department of Biological Sciences, Kean University, Union, New Jersey, USA.

      Recensioner i media

      "...this handbook is of unparalleled value as a reference book and should be on the shelf of all serious investigators in the field... Its value lies in having an immediate reference source at hand, replete with bibliography, for a particular research project, or occasionally for a particularly difficult clinical issue. In these areas, there are simply no competitors in print..." --Clive R. Taylor, Dept of Pathology, The Keck School of Medicine at the University of Southern California, Los Angeles, in APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY (June 2006)"...this book is a valuable reference for medical students, medical technician, pathologists, and clinician alike who are interested in tumor biology or translational research." --HUMAN PATHOLOGY (July 2005)"Volume 1 of this three-volume Elsevier handbook, edited by M.A. Hayat on the 'Immunohistochemistry and in situ Hybridization of Human Carcinomas' is impressive. ...The technical detail included in many contributions surpasses that available from the scientific and clinical journals; all provide a depth of up-to-date knowledge based upon the authors' experience and expertise. Every large hospital and medical school library should be persuaded to buy a copy of this book." --MICRON (2005)"...Professor Hayat’s handbook, Handbook of Immunohistochemistry and in situ Hybridization of Human Carcinomas has the great potential of becoming the handbook of choice for clinician and basic scientists including medical and biological students and biomedical technologists." --Akhouri A. Sinha, Ph.D., Professor of Genetics, Cell Biology and Development, University of Minnesota and Research Scientist at the VA Medical CenterMinneapolis, MN

      Innehållsförteckning

      • Comparison of Immunohistochemistry, in Situ Hybridization, Fluorescence in situ Hybridization, and Chromogenic in situ HybridizationComparison of Chromogenic in situ Hybridization, Fluorescence in situ Hybridization and ImmunohistochemistryTarget and Signal Amplification to Increase the Sensitivity of in situ HybridizationPolymerase Chain ReactionDNA Microarrays TechnologyTissue Microarrays and their Modifications in Highthoroughput Analysis of Clinical SpecimensGene Expression Profiling Using Laser Microdissection in Cancer TissuesDifferential Display of Gene Expression in Human CarcinomasSerial Analysis of Gene Expression in Human DiseasesLung Carcinoma: An IntroductionHitopathological Classification and Phenotype of Lung TumorsImmunohistochemistry and in situ Hybridization of Mucin in Lung CarcinomaImmunohistochemical Expression of MDM2 in Lung CancerImmunohistochemical Expression of E2F1 and p14ARF in Lung CarcinomaRole of Immunohistochemical Expression of Beta-Catenin in Lung CarcinomaImmunohistochemistry of Laminin-5 in Lung CarcinomaRole of Immunohistochemical Expression of Caveolin-1 in Lung CarcinomaRole of Thyroid Transcription Factor-1 in Pulmonary AdenocarcinomaImmunohistochemical and Molecular Pathology of Angiogenesis in Primary Lung AdenocarcinomaImmunohistochemistry of Human Leukocyte Antigen Expression in Lung CarcinomaIn Situ Hybridization and Immunohistochemistry of Telomerase in Lung CarcinomaUse of Fluorescence in situ Hybridization in Detection of Lung Cancer CellsImmunohistochemistry of BCL-2 Gene Expression in Lung CarcinomaBreast Carcinoma: An IntroductionExpression of Vascular Endothelial Growth Factor Receptor-2/Flk-1/KDR in Breast CarcinomaHER2/Neu Amplification and Protein Overexpression in Breast Carcinoma: Immunohistochemistry and Fluorescence in situ HybridizationHer-2/neu Amplification Detected by Fluorescence in situ Hybridization in Cytological Samples from Breast CancerDetection of HER-2 Oncogene with Chromogenic in situ Hybridization in Breast CarcinomaImmunohistochemical Evaluation of Sentinel Lymph Nodes in Breast Carcinoma PatientsCD10 Expression in Normal Breast and Breast Cancer TissueRole of Immunohistochemical Expression of AKT Protein in Breast CarcinomaRole of Immunohistochemical Expression of AKT Protein in Breast CarcinomaImmunohistochemistry of Adhesion Moleculae Ceacam1 Expression in Breast CarcinomaRole of Cadherins in Breast CancerImmunohistochemical Expression of Erythropoitein and Erythropoitin Receptor in Breast CarcinomaLoss of BRCA1 Expression in Breast CarcinomaRole of Immunohistochemical Expression of BRCA1 in Breast CancerFluorescence in situ Hybridization of BRCA1 Gene in Breast CarcinomaImmunohistochemistry of C-MYC Expression in Breast CarcinomaImmunohistochemical Localization of Neuropilin-1 in Human Breast Carcinoma: A Possible Molecular Marker for DiagnosisRole of the Epidermal Growth Factor Receptor in Breast CarcinomaAlterations of the Cell Cycle Regulating Proteins in Invasive Breast CancerCorrelation with Proliferation, Apoptosis, and Clinical OutcomeImmunohistochemistry of Estrogen Receptor Expression in Breast CarcinomaImmunofluorescence and Immunohistochemical Localization of Progesterone Receptors in Breast CarcinomaImmunohistochemical Expression of Cytosolic Thymidine Kinase in Patients with Breast CarcinomaImmunohistochemical Detection of Melanoma Antigen E (MAGE) Expression in Breast CarcinomaRole of Immunohistochemical Expression of Receptors in Male Breast CarcinomaDetection of Glycoconjugates in Breast Cancer Cell Lines: Confocal FluorescenceRole of ETV6-NTRK3 Gene Fusion in Breast CarcinomaRole of CA6 Protein Expression in Breast CarcinomaImmunohistochemistry of EffusionsImmunohistochemistry of Needle Cytopunctures of Breast Carcinomas
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