Cryo-EM Part A: Sample Preparation and Data Collection

Inbunden, Engelska, 2010

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Beskrivning

Cryo-EM Part A: Sample Preparation and Data Collection is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.

Includes time-tested core methods and new innovations applicable to any researcher
Methods included are useful to both established researchers and newcomers to the field
Relevant background and reference information given for procedures can be used as a guide

Produktinformation

Utgivningsdatum:2010-11-22
Mått:152 x 229 x undefined mm
Vikt:880 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Enzymology
Antal sidor:464
Förlag:Elsevier Science
Medarbetare:Grant Jensen
ISBN:9780123749062

Utforska kategorier

Naturvetenskap:allmänt inom Naturvetenskap och teknik

Innehållsförteckning

Historical perspective3D reconstruction from electron micrographs: a personal account of its developmentDavid DeRosier Preparation of 2-D crystalsHenning StahlbergHelical Crystallization of Soluble and Membrane Binding ProteinsElizabeth M. Wilson-Kubalek, Christopher Arthur, Joshua S. ChappiePlunge Freezing for Electron CryomicroscopyAlasdair W. McDowall, Megan J. Dobro, Linda A. Melanson, Grant J. JensenA Practical Guide to the Use of Monolayer Purification and Affinity GridsThomas Walz, Deborah F. Kelly, Danijela DukovskiGraFix: Stabilization of fragile macromolecular complexes for single particle cryo-EMHolger StarkCryo-negative staining of macromolecular assembliesSacha De Carlo, Holger StarkLiposomes on a streptavidin crystal: a system to study membrane proteins by cryo-EMFred J. Sigworth, Liguo WangMicromanipulator-Assisted Vitreous Cryosectioning and Sample Preparation by High-Pressure FreezingMark S. LadinskySite-Specific Biomolecule Labeling with Gold ClustersChristopher J. Ackerson, Richard D. Powell, James F. HainfeldHow to Operate a Cryo-Electron MicroscopeHuilin Li, Jingchuan SunCollecting electron crystallographic data of two-dimensional protein crystalsThomas Walz, Richard K. Hite, Andreas D. Schenk, Zongli Li, Yifan ChengAutomated Data Collection for Electron Microscopic TomographyDavid Agard, Shawn Q. Zheng, J. W. SedatCorrelated Light and Electron CryomicroscopyGrant J. Jensen, Ariane Briegel, Songye Chen, Abraham J. Kosterc, Jürgen M. Plitzkod, Cindi L. SchwartzPhase Plates for Transmission Electron MicroscopyRadostin Danev, Kuniaki NagayamaRadiation damage in electron cryomicroscopy John L. Rubinstein, Lindsay A. Baker