Cilia: Motors and Regulation

Inbunden, Engelska, 2009

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Beskrivning

Along with its companion volume on axonemal dynein-mediated motility, this book provides researchers with a comprehensive and up-to-date source of methods for the analysis cilia and flagella, focusing primarily on approaches that have been devised or significantly extended since the last volume of Methods in Cell Biology on this topic (volume 47, 1995). Edited by Stephen M. King and Gregory J. Pazour, the newest installment of this highly acclaimed serial will serve as an essential addition to the study of cilia and flagella.

Covers protocols for cilia and flagella across systems and species
Both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time
Relevant to clinicians interested in respiratory disease, male infertility, and other syndromes who need to learn biochemical, molecular, and genetic approaches to studying cilia, flagella, and related structures

Produktinformation

Utgivningsdatum:2009-12-05
Mått:191 x 235 x undefined mm
Vikt:640 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Cell Biology
Antal sidor:400
Förlag:Elsevier Science
ISBN:9780123749741

Utforska kategorier

Cellbiologi inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik

Mer om författaren

Stephen M. King is Professor of Molecular Biology and Biophysics at the University of Connecticut School of Medicine and is also director of the electron microscopy facility. He has studied the structure, function and regulation of dyneins for over 30 years using a broad array of methodologies including classical/molecular genetics, protein biochemistry, NMR structural biology and molecular modeling, combined with cell biological approaches, imaging and physiological measurements.

Innehållsförteckning

MCB, 921. Bioinformatic Approaches to Dynein Heavy Chain ClassificationToshiki Yagi2. Identification and Characterization of Dynein Genes in TetrahymenaDavid E. Wilkes, Nicole Bennardo, Clarence W.C. Chan, Yu-Loung Chang,Elizabeth O. Corpuz, Jennifer DuMond, Jordan A. Eboreime, Julianna Erickson, Jonathan Hetzel, Erin E. Heyer, Mark J. Hubenschmidt, Ekaterina Kniazeva, Hallie Kuhn, Michelle Lum, Andrea Sand, Alicia Schep, Oksana Sergeeva, Natt Supab, Caroline R. Townsend, Liesl Van Ryswyk, Hadley E. Watson, Alice E. Wiedeman, Vidyalakshmi Rajagopalan, and David J. Asai3. Purification of Axonemal Dyneins and Dynein-Associated Componentsfrom ChlamydomonasStephen M. King4. Purification of Dyneins from Sperm FlagellaKazuo Inaba and Katsutoshi Mizuno5. Protein Engineering Approaches to Study the Dynein Mechanism using a Dictyostelium Expression SystemTakahide Kon, Tomohiro Shima, and Kazuo Sutoh6. Biophysical Measurements on Axonemal DyneinsHiroaki Kojima, Shiori Toba, Hitoshi Sakakibara, and Kazuhiro Oiwa7. Protein Electroporation into Chlamydomonas for Mutant RescueMasahito Hayashi and Ritsu Kamiya8. Analysis of the Role of Nucleotides in Axonemal Dynein FunctionChikako Shingyoji9. The Regulation of Dynein-Driven Microtubule Sliding in ChlamydomonasFlagella by Axonemal Kinases and PhosphatasesCandice A. Elam, Winfield S. Sale, and Maureen Wirschell10. Analysis of Redox-Sensitive Dynein ComponentsKen-ichi Wakabayashi11. Calcium Regulation of Ciliary Motility: Analysis of Axonemal Calcium-Binding ProteinsChristen DiPetrillo and Elizabeth Smith12. Isolation and Analysis of Radial Spoke ProteinsPooja Kelekar, Mei Wei, and Pinfen Yang13. Analysis of the Central Pair Microtubule Complex in Chlamydomonas reinhardtiiDavid R. Mitchell and Brandon Smith