Microtubules: in vivo

Inbunden, Engelska, 2010

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Beskrivning

Microtubules: in vivo includes chapters by experts around the world on many aspects of microtubule imaging in living and fixed cells; assays to study microtubule function in a wide array of model organisms and cultured cells; high resolution approaches to study of the cytoskeleton. The authors share their years of experience, outlining potential pitfalls and critical factors to consider in experimental design, experimental implementation and data interpretation.

Includes chapters by experts around the world on many aspects of microtubule imaging in living and fixed cells; assays to study microtubule function in a wide array of model organisms and cultured cells; high resolution approaches to study of the cytoskeleton
The authors share their years of experience, outlining potential pitfalls and critical factors to consider in experimental design, experimental implementation and data interpretation

Produktinformation

Utgivningsdatum:2010-09-24
Mått:191 x 235 x undefined mm
Vikt:1 030 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Cell Biology
Antal sidor:552
Förlag:Elsevier Science
ISBN:9780123813497

Utforska kategorier

Cellbiologi inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik

Mer om författaren

University of Pennsylvania, Philadelphia, USA and Institut Curie, Paris, France

Innehållsförteckning

Microtubule dynamics at the cell cortex probed by TIRF microscopyIlya Grigoriev and Anna AkhmanovaMicroscopy methods for the study of centriole biogenesis and function in DrosophilaAna Rodrigues Martins, Pedro Machado, Giuliano Callaini and Monica Bettencourt-DiasQuality control in single molecule studies of kinesins and microtubule-associated proteinsGary J. BrouhardImaging cilia in zebrafishKimberly M. Jaffe, Stephan Y. Thiberge, Margaret E. Bisher and Rebecca D. BurdineAutomatic Identification of Microtubules in Cellular Electron TomographyDaniyar Nurgaliev, Timur Gatanov and Daniel J. NeedlemanImaging and analysis of the microtubule cytoskeleton in GiardiaScott C. Dawson and Susan A. HouseKinetochore-microtubule dynamics and attachment stabilityJennifer G. DeLucaAssessment of mitotic spindle phenotypes in Drosophila S2 cellsGohta GoshimaLive cell imaging techniques for analyses of microtubules in DictyosteliumMatthias Samereier, Irene Meyer, Michael P. Koonce and Ralph GräfMicrotubule dynamics in dendritic spinesLukas C. Kapitein , Kah Wai Yau and Casper C. HoogenraadAnalysis of Microtubules in Budding YeastAlexander Rauch, Elena Nazarova and Jackie VogelDetermination of Microtubule Dynamic Instability in Living CellsKathy Kamath, Emin Oroudjev, and Mary Ann JordanModeling microtubule-mediated forces and centrosome positioning in Caenorhabditis elegans embryosAkatsuki Kimura1 and Shuichi OnamiCryo electron tomography of cellular microtubulesRoman I. KoningMicrotubule dynamics in plant cellsHenrik Buschmann, Adrian Sambade, Edouard Pesquet, Grant Calder and Clive W. LloydDrosophila S2 cells as a model system to investigate mitotic spindle dynamics, architecture and functionSara Moutinho-Pereira, Irina Matos and Helder MaiatoProtein micropatterns: a direct printing protocol using deep UVsAmmar Azioune, Nicolas Carpi, Manuel Théry and Matthieu PielOptical trapping and laser ablation of microtubules in fission yeastNicola Maghelli and Iva M. Tolić-NørrelykkeMelanophores for Microtubule dynamics and motility assaysKazuho Ikeda, Irina Semenova, Olga Zhapparova, and Vladimir RodionovNew and old reagents for fluorescent-protein tagging of microtubules in fission yeast: experimental and critical evaluationHilary A. Snaith, Andreas Anders, Itaru Samejima and Kenneth E. SawinImaging of mitotic spindle dynamics in C. elegans embryosMika Toya, Yumi Iida and Asako SugimotoA fast microfluidic temperature control device for studying microtubule dynamics in fission yeastGuilhem Velve-Casquillas, Judite Costa, Frédérique Carlier-Grynkorn , Adeline Mayeux and Phong T. TranMicrotubule-dependent spatial organization of mitochondria in fission yeastMaitreyi Das, Stephan Chiron and Fulvia VerdePhotoactivatable GFP-TubulinU. Serdar Tulu*, Nick P. FerenzThe Use of Fluorescence Redistribution After Photobleaching for Analysis of Cellular Microtubule DynamicsClaire E. Walczak, Rania S. Rizk and Sidney L. ShawAnalysis of microtubule polymerization dynamics in live cells Sarah Gierke, Praveen Kumar, and Torsten Wittmann