Building a Cell from its Component Parts

Wallace Marshall is an electrical engineer by training, who became interested in biology out of a desire to understand how cells solve engineering problems, such as determining the size of organelles. He received his Ph.D. at UCSF with John Sedat, where he studied the diffusional of motion of interphase chromatin using live cell imaging and computational image analysis. He then trained as a postdoc with Joel Rosenbaum at Yale, where he began studying the mechanisms regulating the length of cilia and flagella. He is now Profess of Biochemistry at UCSF, where he lab continues to study the assembly and length regulation of cilia and flagella, as well as the mechanisms that regulate the size of other organelles. His work takes advantage of an integrated combination of methods including genetics, microscopy, and computational modeling, as well as a wide variety of model organisms including Chlamydomonas reinhardtii, Stentor coeruleus, yeast, flatworms, and mammalian cells.

• 1. In Vitro Systems for the Study of Microtubule-Based Cell Polarity in Fission YeastNúria Taberner, Andries Lof, Sophie Roth, Dimitry Lamers, Hans Zeijlemaker and Marileen Dogterom2. Microtubules, MAPs, and Motor PatternsKasimira T. Stanhope and Jennifer L. Ross3. Self-Organization of Motors and Microtubules in Lipid-Monolayered DropletsHella Baumann and Thomas Surrey4. Reconstitution of Microtubule Based Motility Using Cell ExtractsSwathi Ayloo and Erika L. F. Holzbaur5. Building Cells for Quantitative, Live-cell Analyses of Collective Motor Protein FunctionsEric A. Kumar, David Tsao, Anand Radhakrishnan and Michael Diehl6. Reconstituting Cytoskeletal Contraction Events with Biomimetic Actin-Myosin Active gelsJosé Alvarado and Gijsje H. Koenderink7. Building an Artificial Actin Cortex on Microscopic Pillar ArraysR. Ayadi and W. H. Roos8. Triggering Actin Polymerization in Xenopus Egg Extracts from Phosphoinositide-Containing Lipid BilayersAstrid Walrant, Daniel S. Saxton, Guilherme Pereira Correia and Jennifer L. Gallop9. Reconstituting Geometry Modulated Protein Patterns in Membrane CompartmentsKatja Zieske and Petra Schwille10. Structural and Functional Studies of Membrane Remodeling MachinesRaghav Kalia, Nathaniel Talledge and Adam Frost11. Building Interconnected Membrane NetworksMatthew A. Holden12. Using Supported Bilayers to Study the Spatiotemporal Organization of Membrane Bound ProteinsPhuong A. Nguyen, Christine M. Field, Aaron C. Groen, Timothy J. Mitchison and Martin Loose 13. Reconstituting ParA/ParB-Mediated Transport of DNA CargoAnthony G. Vecchiarelli, James A. Taylor and Kiyoshi Mizuuchi14. Cell-Sized Liposomes that Mimic Cell Motility and the Cell CortexJoël Lemière, Kevin Carvalho and Cécile Sykes15. Reconstitution of Cortical Actin Networks Within Water-In-Oil EmulsionsEnas Abu Shah, Maya Malik-Garbi and Kinneret Keren16. Engineering Artificial Cells by Combining HeLa-Based Cell-Free Expression and Ultra-Thin Double Emulsion TemplateKenneth K.Y. Ho, Victoria L. Murray, Allen P. Liu17. Reconstitution of Proteins on Electroformed Giant Unilamellar VesiclesEva M. Schmid, David L. Richmond, and Daniel A. Fletcher18. Reconstituting SNARE-Mediated Membrane Fusion at the Single Liposome LevelVolker Kiessling, Binyong Liang and Lukas K. Tamm