Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla’s research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.

Praise for the Series:"Should be on the shelves of all libraries in the world as a whole collection." --CHEMISTRY IN INDUSTRY"The work most often consulted in the lab." --ENZYMOLOGIA"The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE

• 1. Targeted m6A reader proteins to study the epitranscriptomeSimone Rauch and Bryan C. Dickinson2. Synthetic gene expression circuits regulating sexual reproductionNobuo Fukuda and Shinya Honda3. Nucleotide resolution sequencing of N4-acetylcytidine in RNAJustin M. Thomas, Keri M. Bryson and Jordan L. Meier4. A guide for drug inducible genome editing with HIT systemsChen Zhao, Shixian Wei and Yu Wang5. A guide for drug inducible transcriptional activation with HIT systemsChen Zhao, Shixian Wei and Yu Wang6. Direct cloning and heterologous expression of natural product biosynthetic gene clusters by transformation-associated recombinationJia Jia Zhang, Kazuya Yamanaka, Xiaoyu Tang and Bradley S. Moore7. Salt-sensitive intein for large-scale polypeptide productionYi-Zong Lee and Shih-Che Sue8. Methods for the recombinant expression of active tyrosine kinase domains: Guidelines and pitfallsM. Escarlet Díaz Galicia, Abdullah Aldehaiman, SeungBeom Hong, Stefan T. Arold and Raik Grünberg9. Design, cloning and characterization of transcription factor-based inducible gene expression systemsErik K.R. Hanko, Nigel P. Minton and Naglis Malys10. Design, construction, and validation of optogenetic proteinsColin P. O'Banion, Anwesha Goswami and David S. Lawrence11. Overcoming component limitations in synthetic biology through transposon-mediated protein engineeringJoshua T. Atkinson, Bingyan Wu, Laura Segatori and Jonathan J. Silberg12. Chemical biology of glycoproteins: From chemical synthesis to biological impactYaohao Li, Amy H. Tran, Samuel J. Danishefsky and Zhongping Tan13. In bulla functional channel expression systems that mimic bacterial synthetic membranesMasayuki Iwamoto and Shigetoshi Oiki14. A mass spectrometry-based isotope-coded mass tag method to map thiol accessibility in biological systemsJohn E. Gadbery and Nicole S. Sampson15. Quick-soaking of crystals reveals unprecedented insights into the catalytic mechanism of glycosyltransferasesDavid Albesa-Jové, Javier O. Cifuente, Beatriz Trastoy and Marcelo E. Guerin16. Cysteine-ethylation of tissue-extracted membrane proteins as a tool to detect conformational states by solid-state NMR spectroscopyDaniel K. Weber, Taysir Bader, Erik K. Larsen, Songlin Wang, Tata Gopinath, Mark Distefano and Gianluigi Veglia17. Improved sensitivity and resolution of in-cell NMR spectraDavid S. Burz, Leonard Breindel and Alexander Shekhtman18. High-throughput methods in aptamer discovery and analysisKyle H. Cole and Andrej Lupták19. Drop-in-well chamber for droplet interface bilayer with built-in electrodesKazuhiro Urakubo, Masayuki Iwamoto and Shigetoshi Oiki

Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla’s research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.

Praise for the Series:"Should be on the shelves of all libraries in the world as a whole collection." --CHEMISTRY IN INDUSTRY"The work most often consulted in the lab." --ENZYMOLOGIA"The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE

• 1. Targeted m6A reader proteins to study the epitranscriptomeSimone Rauch and Bryan C. Dickinson2. Synthetic gene expression circuits regulating sexual reproductionNobuo Fukuda and Shinya Honda3. Nucleotide resolution sequencing of N4-acetylcytidine in RNAJustin M. Thomas, Keri M. Bryson and Jordan L. Meier4. A guide for drug inducible genome editing with HIT systemsChen Zhao, Shixian Wei and Yu Wang5. A guide for drug inducible transcriptional activation with HIT systemsChen Zhao, Shixian Wei and Yu Wang6. Direct cloning and heterologous expression of natural product biosynthetic gene clusters by transformation-associated recombinationJia Jia Zhang, Kazuya Yamanaka, Xiaoyu Tang and Bradley S. Moore7. Salt-sensitive intein for large-scale polypeptide productionYi-Zong Lee and Shih-Che Sue8. Methods for the recombinant expression of active tyrosine kinase domains: Guidelines and pitfallsM. Escarlet Díaz Galicia, Abdullah Aldehaiman, SeungBeom Hong, Stefan T. Arold and Raik Grünberg9. Design, cloning and characterization of transcription factor-based inducible gene expression systemsErik K.R. Hanko, Nigel P. Minton and Naglis Malys10. Design, construction, and validation of optogenetic proteinsColin P. O'Banion, Anwesha Goswami and David S. Lawrence11. Overcoming component limitations in synthetic biology through transposon-mediated protein engineeringJoshua T. Atkinson, Bingyan Wu, Laura Segatori and Jonathan J. Silberg12. Chemical biology of glycoproteins: From chemical synthesis to biological impactYaohao Li, Amy H. Tran, Samuel J. Danishefsky and Zhongping Tan13. In bulla functional channel expression systems that mimic bacterial synthetic membranesMasayuki Iwamoto and Shigetoshi Oiki14. A mass spectrometry-based isotope-coded mass tag method to map thiol accessibility in biological systemsJohn E. Gadbery and Nicole S. Sampson15. Quick-soaking of crystals reveals unprecedented insights into the catalytic mechanism of glycosyltransferasesDavid Albesa-Jové, Javier O. Cifuente, Beatriz Trastoy and Marcelo E. Guerin16. Cysteine-ethylation of tissue-extracted membrane proteins as a tool to detect conformational states by solid-state NMR spectroscopyDaniel K. Weber, Taysir Bader, Erik K. Larsen, Songlin Wang, Tata Gopinath, Mark Distefano and Gianluigi Veglia17. Improved sensitivity and resolution of in-cell NMR spectraDavid S. Burz, Leonard Breindel and Alexander Shekhtman18. High-throughput methods in aptamer discovery and analysisKyle H. Cole and Andrej Lupták19. Drop-in-well chamber for droplet interface bilayer with built-in electrodesKazuhiro Urakubo, Masayuki Iwamoto and Shigetoshi Oiki

Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla’s research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.

Praise for the Series:"Should be on the shelves of all libraries in the world as a whole collection." --CHEMISTRY IN INDUSTRY"The work most often consulted in the lab." --ENZYMOLOGIA"The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE

• 1. Targeted m6A reader proteins to study the epitranscriptomeSimone Rauch and Bryan C. Dickinson2. Synthetic gene expression circuits regulating sexual reproductionNobuo Fukuda and Shinya Honda3. Nucleotide resolution sequencing of N4-acetylcytidine in RNAJustin M. Thomas, Keri M. Bryson and Jordan L. Meier4. A guide for drug inducible genome editing with HIT systemsChen Zhao, Shixian Wei and Yu Wang5. A guide for drug inducible transcriptional activation with HIT systemsChen Zhao, Shixian Wei and Yu Wang6. Direct cloning and heterologous expression of natural product biosynthetic gene clusters by transformation-associated recombinationJia Jia Zhang, Kazuya Yamanaka, Xiaoyu Tang and Bradley S. Moore7. Salt-sensitive intein for large-scale polypeptide productionYi-Zong Lee and Shih-Che Sue8. Methods for the recombinant expression of active tyrosine kinase domains: Guidelines and pitfallsM. Escarlet Díaz Galicia, Abdullah Aldehaiman, SeungBeom Hong, Stefan T. Arold and Raik Grünberg9. Design, cloning and characterization of transcription factor-based inducible gene expression systemsErik K.R. Hanko, Nigel P. Minton and Naglis Malys10. Design, construction, and validation of optogenetic proteinsColin P. O'Banion, Anwesha Goswami and David S. Lawrence11. Overcoming component limitations in synthetic biology through transposon-mediated protein engineeringJoshua T. Atkinson, Bingyan Wu, Laura Segatori and Jonathan J. Silberg12. Chemical biology of glycoproteins: From chemical synthesis to biological impactYaohao Li, Amy H. Tran, Samuel J. Danishefsky and Zhongping Tan13. In bulla functional channel expression systems that mimic bacterial synthetic membranesMasayuki Iwamoto and Shigetoshi Oiki14. A mass spectrometry-based isotope-coded mass tag method to map thiol accessibility in biological systemsJohn E. Gadbery and Nicole S. Sampson15. Quick-soaking of crystals reveals unprecedented insights into the catalytic mechanism of glycosyltransferasesDavid Albesa-Jové, Javier O. Cifuente, Beatriz Trastoy and Marcelo E. Guerin16. Cysteine-ethylation of tissue-extracted membrane proteins as a tool to detect conformational states by solid-state NMR spectroscopyDaniel K. Weber, Taysir Bader, Erik K. Larsen, Songlin Wang, Tata Gopinath, Mark Distefano and Gianluigi Veglia17. Improved sensitivity and resolution of in-cell NMR spectraDavid S. Burz, Leonard Breindel and Alexander Shekhtman18. High-throughput methods in aptamer discovery and analysisKyle H. Cole and Andrej Lupták19. Drop-in-well chamber for droplet interface bilayer with built-in electrodesKazuhiro Urakubo, Masayuki Iwamoto and Shigetoshi Oiki