Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla’s research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.

Praise for the Series:"Should be on the shelves of all libraries in the world as a whole collection." --CHEMISTRY IN INDUSTRY"The work most often consulted in the lab." --ENZYMOLOGIA"The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE

• 1. Input-dependent post-translational control of the reporter output enhances dynamic resolution of mammalian signaling systemsBrianna E.K. Jayanthi, Wenting Zhao and Laura Segatori2. Design and synthesis of fluorescent activity probes for protein phosphatasesGarrett R. Casey, Jon R. Beck and Cliff I. Stains3. Utilizing split-NanoLuc luciferase fragments as luminescent probes for protein solubility in living cellsTravis J. Nelson, Jia Zhao and Cliff I. Stains4. Engineering of a sulfotyrosine-recognizing small protein scaffold for the study of protein tyrosine O-sulfationJustin Lawrie, Wei Niu and Jiantao Guo5. Synthesis and screening of bead-displayed combinatorial librariesTodd M. Doran, Paige Dickson, John Maina Ndungu, Peng Ge, Irena Suponitsky-Kroyter, Hongchan An and Thomas Kodadek6. Visualizing the brain's astrocytesAlyssa N. Preston, Danielle A. Cervasio and Scott T. Laughlin7. Modular activatable bioorthogonal reagentsPratik Kumar and Scott T. Laughlin8. Engineering PEG-fatty acid stapled, long-acting peptide agonists for G protein-coupled receptorsSam Lear, Zaid Amso and Weijun Shen9. Quantifying drug-target engagement in live cells using sulfonyl fluoride chemical probesLyn H. Jones, Hua Xu and Olugbeminiyi O. Fadeyi10. Selection and optimization of enzyme reporters for chemical cytometryAngela Proctor, Qunzhao Wang, David S. Lawrence and Nancy L. Allbritton11. Synthetic cell-like membrane interfaces for probing dynamic protein–lipid interactionsSpencer T. Glantz, Erin E. Berlew and Brian Y. Chow12. Rational engineering acyltransferase domain of modular polyketide synthase for expanding substrate specificityWan Zhang, Linjun Zhou, Chunyu Li, Zixin Deng and Xudong Qu13. Identifying potentially O-GlcNAcylated proteins using metabolic labeling, bioorthogonal enrichment, and Western blottingNarek Darabedian and Matthew R. Pratt14. Optogenetic perturbation of the biochemical pathways that control cell behaviorLauren L. Haar, David S. Lawrence and Robert M. Hughes15. pH-shift stress on antibodiesHiroshi Imamura and Shinya Honda16. Small molecule target identification using photo-affinity chromatographySeung-Yong Seo and Timothy W. Corson17. Computational structural enzymology methodologies for the study and engineering of fatty acid synthases, polyketide synthases and nonribosomal peptide synthetasesAndrew J. Schaub, Gabriel O. Moreno, Shiji Zhao, Hau V. Truong, Ray Luo and Shiou-Chuan Sheryl Tsai18. On-cell coordination chemistry: Chemogenetic activation of membrane-bound glutamate receptors in living cellsRyou Kubota, Shigeki Kiyonaka and Itaru Hamachi19. Bioorthogonal oncometabolite ligationChloe A. Briney, Susana Najera and Jordan L. Meier

Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla’s research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.

Praise for the Series:"Should be on the shelves of all libraries in the world as a whole collection." --CHEMISTRY IN INDUSTRY"The work most often consulted in the lab." --ENZYMOLOGIA"The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE

• 1. Input-dependent post-translational control of the reporter output enhances dynamic resolution of mammalian signaling systemsBrianna E.K. Jayanthi, Wenting Zhao and Laura Segatori2. Design and synthesis of fluorescent activity probes for protein phosphatasesGarrett R. Casey, Jon R. Beck and Cliff I. Stains3. Utilizing split-NanoLuc luciferase fragments as luminescent probes for protein solubility in living cellsTravis J. Nelson, Jia Zhao and Cliff I. Stains4. Engineering of a sulfotyrosine-recognizing small protein scaffold for the study of protein tyrosine O-sulfationJustin Lawrie, Wei Niu and Jiantao Guo5. Synthesis and screening of bead-displayed combinatorial librariesTodd M. Doran, Paige Dickson, John Maina Ndungu, Peng Ge, Irena Suponitsky-Kroyter, Hongchan An and Thomas Kodadek6. Visualizing the brain's astrocytesAlyssa N. Preston, Danielle A. Cervasio and Scott T. Laughlin7. Modular activatable bioorthogonal reagentsPratik Kumar and Scott T. Laughlin8. Engineering PEG-fatty acid stapled, long-acting peptide agonists for G protein-coupled receptorsSam Lear, Zaid Amso and Weijun Shen9. Quantifying drug-target engagement in live cells using sulfonyl fluoride chemical probesLyn H. Jones, Hua Xu and Olugbeminiyi O. Fadeyi10. Selection and optimization of enzyme reporters for chemical cytometryAngela Proctor, Qunzhao Wang, David S. Lawrence and Nancy L. Allbritton11. Synthetic cell-like membrane interfaces for probing dynamic protein–lipid interactionsSpencer T. Glantz, Erin E. Berlew and Brian Y. Chow12. Rational engineering acyltransferase domain of modular polyketide synthase for expanding substrate specificityWan Zhang, Linjun Zhou, Chunyu Li, Zixin Deng and Xudong Qu13. Identifying potentially O-GlcNAcylated proteins using metabolic labeling, bioorthogonal enrichment, and Western blottingNarek Darabedian and Matthew R. Pratt14. Optogenetic perturbation of the biochemical pathways that control cell behaviorLauren L. Haar, David S. Lawrence and Robert M. Hughes15. pH-shift stress on antibodiesHiroshi Imamura and Shinya Honda16. Small molecule target identification using photo-affinity chromatographySeung-Yong Seo and Timothy W. Corson17. Computational structural enzymology methodologies for the study and engineering of fatty acid synthases, polyketide synthases and nonribosomal peptide synthetasesAndrew J. Schaub, Gabriel O. Moreno, Shiji Zhao, Hau V. Truong, Ray Luo and Shiou-Chuan Sheryl Tsai18. On-cell coordination chemistry: Chemogenetic activation of membrane-bound glutamate receptors in living cellsRyou Kubota, Shigeki Kiyonaka and Itaru Hamachi19. Bioorthogonal oncometabolite ligationChloe A. Briney, Susana Najera and Jordan L. Meier

Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla’s research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.

Praise for the Series:"Should be on the shelves of all libraries in the world as a whole collection." --CHEMISTRY IN INDUSTRY"The work most often consulted in the lab." --ENZYMOLOGIA"The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE

• 1. Input-dependent post-translational control of the reporter output enhances dynamic resolution of mammalian signaling systemsBrianna E.K. Jayanthi, Wenting Zhao and Laura Segatori2. Design and synthesis of fluorescent activity probes for protein phosphatasesGarrett R. Casey, Jon R. Beck and Cliff I. Stains3. Utilizing split-NanoLuc luciferase fragments as luminescent probes for protein solubility in living cellsTravis J. Nelson, Jia Zhao and Cliff I. Stains4. Engineering of a sulfotyrosine-recognizing small protein scaffold for the study of protein tyrosine O-sulfationJustin Lawrie, Wei Niu and Jiantao Guo5. Synthesis and screening of bead-displayed combinatorial librariesTodd M. Doran, Paige Dickson, John Maina Ndungu, Peng Ge, Irena Suponitsky-Kroyter, Hongchan An and Thomas Kodadek6. Visualizing the brain's astrocytesAlyssa N. Preston, Danielle A. Cervasio and Scott T. Laughlin7. Modular activatable bioorthogonal reagentsPratik Kumar and Scott T. Laughlin8. Engineering PEG-fatty acid stapled, long-acting peptide agonists for G protein-coupled receptorsSam Lear, Zaid Amso and Weijun Shen9. Quantifying drug-target engagement in live cells using sulfonyl fluoride chemical probesLyn H. Jones, Hua Xu and Olugbeminiyi O. Fadeyi10. Selection and optimization of enzyme reporters for chemical cytometryAngela Proctor, Qunzhao Wang, David S. Lawrence and Nancy L. Allbritton11. Synthetic cell-like membrane interfaces for probing dynamic protein–lipid interactionsSpencer T. Glantz, Erin E. Berlew and Brian Y. Chow12. Rational engineering acyltransferase domain of modular polyketide synthase for expanding substrate specificityWan Zhang, Linjun Zhou, Chunyu Li, Zixin Deng and Xudong Qu13. Identifying potentially O-GlcNAcylated proteins using metabolic labeling, bioorthogonal enrichment, and Western blottingNarek Darabedian and Matthew R. Pratt14. Optogenetic perturbation of the biochemical pathways that control cell behaviorLauren L. Haar, David S. Lawrence and Robert M. Hughes15. pH-shift stress on antibodiesHiroshi Imamura and Shinya Honda16. Small molecule target identification using photo-affinity chromatographySeung-Yong Seo and Timothy W. Corson17. Computational structural enzymology methodologies for the study and engineering of fatty acid synthases, polyketide synthases and nonribosomal peptide synthetasesAndrew J. Schaub, Gabriel O. Moreno, Shiji Zhao, Hau V. Truong, Ray Luo and Shiou-Chuan Sheryl Tsai18. On-cell coordination chemistry: Chemogenetic activation of membrane-bound glutamate receptors in living cellsRyou Kubota, Shigeki Kiyonaka and Itaru Hamachi19. Bioorthogonal oncometabolite ligationChloe A. Briney, Susana Najera and Jordan L. Meier