Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla’s research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.

"Given that the preceding Parts A and B of this series were both published nearly a decade ago (i.e., 2011), Part C offers a timely overview of recent technological advances and novel findings in these rapidly moving areas of research and development. This most recent addition to the prestigious Methods in Enzymology series complements, rather than competes with, the numerous books available on these topics, which generally focus on either synthetic or chemical biology or specific aspects thereof (e.g., "Inorganic Chemical Biology" or "Systems Biology Application in Synthetic Biology"), but not both, and rarely offer the detailed descriptions of specific methods and procedures found in this book." --Doody

• 1. Competitive binding assay for biotin and biotin derivatives, based on avidin and biotin-4-fluoresceinElke Oberbichler, Maria Wiesauer, Eva Schlögl, Jessica Stangl, Felix Faschinger, Günther Knör, Hermann J. Gruber and Vesa P. Hytönen2. (Strept)avidin as a template for ligands other than biotin: An overviewVesa P. Hytönen3. Engineering siderophoresSina Rütschlin and Thomas Böttcher4. Competitive profiling for enzyme inhibitors using chemical probesMichaela Prothiwa and Thomas Böttcher5. The NMR tube bioreactorAlexandra V. Chatzikonstantinou, Antonis Tsiailanis, Ioannis P. Gerothanassis, Haralambos Stamatis, Enrico Ravera, Marco Fragai, Claudio Luchinat, Giacomo Parigi and Andreas G. Tzakos6. A chemically-controlled system for activating RAS GTPasesEmily M. Dieter and Dustin J. Maly7. Temporal and rheostatic control of genome editing with a chemically-inducible Cas9Cindy T. Wei, Dustin J. Maly and Douglas M. Fowler8. Synthetic receptors to understand and control cellular functionsHung-Ju Chang and Jerome Bonnet9. A suite of bioassays to evaluate CREB inhibitorsBingbing X. Li and Xiangshu Xiao10. Identification of lamins as the molecular targets of LBL1 using a clickable photoaffinity probeXiangshu Xiao and Bingbing X. Li11. REX technologies for profiling and decoding the electrophile signaling axes mediated by Rosetta Stone proteinsMarcus J. C. Long, Daniel A. Urul and Yimon Aye12. Building artificial genetic circuits to understand protein functionLouis H. Scott, James C. Mathews, Aleksandra Filipovska and Oliver Rackham13. Methods for studying human sirtuins with activity-based chemical probesSong Zheng, Jessica Wohlfahrt, Ian Cohen and Yana Cen14. Site-directed labeling of β-arrestin with monobromobimane for measuring their interaction with G protein-coupled receptorsAshish Srivastava, Mithu Baidya, Hemlata Dwivedi-Agnihotri and Arun K. Shukla15. Reversible biotinylation of purified proteins for measuring protein-protein interactionsHemlata Dwivedi-Agnihotri, Ashish Srivastava and Arun K. Shukla

Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla’s research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.

"Given that the preceding Parts A and B of this series were both published nearly a decade ago (i.e., 2011), Part C offers a timely overview of recent technological advances and novel findings in these rapidly moving areas of research and development. This most recent addition to the prestigious Methods in Enzymology series complements, rather than competes with, the numerous books available on these topics, which generally focus on either synthetic or chemical biology or specific aspects thereof (e.g., "Inorganic Chemical Biology" or "Systems Biology Application in Synthetic Biology"), but not both, and rarely offer the detailed descriptions of specific methods and procedures found in this book." --Doody

• 1. Competitive binding assay for biotin and biotin derivatives, based on avidin and biotin-4-fluoresceinElke Oberbichler, Maria Wiesauer, Eva Schlögl, Jessica Stangl, Felix Faschinger, Günther Knör, Hermann J. Gruber and Vesa P. Hytönen2. (Strept)avidin as a template for ligands other than biotin: An overviewVesa P. Hytönen3. Engineering siderophoresSina Rütschlin and Thomas Böttcher4. Competitive profiling for enzyme inhibitors using chemical probesMichaela Prothiwa and Thomas Böttcher5. The NMR tube bioreactorAlexandra V. Chatzikonstantinou, Antonis Tsiailanis, Ioannis P. Gerothanassis, Haralambos Stamatis, Enrico Ravera, Marco Fragai, Claudio Luchinat, Giacomo Parigi and Andreas G. Tzakos6. A chemically-controlled system for activating RAS GTPasesEmily M. Dieter and Dustin J. Maly7. Temporal and rheostatic control of genome editing with a chemically-inducible Cas9Cindy T. Wei, Dustin J. Maly and Douglas M. Fowler8. Synthetic receptors to understand and control cellular functionsHung-Ju Chang and Jerome Bonnet9. A suite of bioassays to evaluate CREB inhibitorsBingbing X. Li and Xiangshu Xiao10. Identification of lamins as the molecular targets of LBL1 using a clickable photoaffinity probeXiangshu Xiao and Bingbing X. Li11. REX technologies for profiling and decoding the electrophile signaling axes mediated by Rosetta Stone proteinsMarcus J. C. Long, Daniel A. Urul and Yimon Aye12. Building artificial genetic circuits to understand protein functionLouis H. Scott, James C. Mathews, Aleksandra Filipovska and Oliver Rackham13. Methods for studying human sirtuins with activity-based chemical probesSong Zheng, Jessica Wohlfahrt, Ian Cohen and Yana Cen14. Site-directed labeling of β-arrestin with monobromobimane for measuring their interaction with G protein-coupled receptorsAshish Srivastava, Mithu Baidya, Hemlata Dwivedi-Agnihotri and Arun K. Shukla15. Reversible biotinylation of purified proteins for measuring protein-protein interactionsHemlata Dwivedi-Agnihotri, Ashish Srivastava and Arun K. Shukla

Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla’s research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.

"Given that the preceding Parts A and B of this series were both published nearly a decade ago (i.e., 2011), Part C offers a timely overview of recent technological advances and novel findings in these rapidly moving areas of research and development. This most recent addition to the prestigious Methods in Enzymology series complements, rather than competes with, the numerous books available on these topics, which generally focus on either synthetic or chemical biology or specific aspects thereof (e.g., "Inorganic Chemical Biology" or "Systems Biology Application in Synthetic Biology"), but not both, and rarely offer the detailed descriptions of specific methods and procedures found in this book." --Doody

• 1. Competitive binding assay for biotin and biotin derivatives, based on avidin and biotin-4-fluoresceinElke Oberbichler, Maria Wiesauer, Eva Schlögl, Jessica Stangl, Felix Faschinger, Günther Knör, Hermann J. Gruber and Vesa P. Hytönen2. (Strept)avidin as a template for ligands other than biotin: An overviewVesa P. Hytönen3. Engineering siderophoresSina Rütschlin and Thomas Böttcher4. Competitive profiling for enzyme inhibitors using chemical probesMichaela Prothiwa and Thomas Böttcher5. The NMR tube bioreactorAlexandra V. Chatzikonstantinou, Antonis Tsiailanis, Ioannis P. Gerothanassis, Haralambos Stamatis, Enrico Ravera, Marco Fragai, Claudio Luchinat, Giacomo Parigi and Andreas G. Tzakos6. A chemically-controlled system for activating RAS GTPasesEmily M. Dieter and Dustin J. Maly7. Temporal and rheostatic control of genome editing with a chemically-inducible Cas9Cindy T. Wei, Dustin J. Maly and Douglas M. Fowler8. Synthetic receptors to understand and control cellular functionsHung-Ju Chang and Jerome Bonnet9. A suite of bioassays to evaluate CREB inhibitorsBingbing X. Li and Xiangshu Xiao10. Identification of lamins as the molecular targets of LBL1 using a clickable photoaffinity probeXiangshu Xiao and Bingbing X. Li11. REX technologies for profiling and decoding the electrophile signaling axes mediated by Rosetta Stone proteinsMarcus J. C. Long, Daniel A. Urul and Yimon Aye12. Building artificial genetic circuits to understand protein functionLouis H. Scott, James C. Mathews, Aleksandra Filipovska and Oliver Rackham13. Methods for studying human sirtuins with activity-based chemical probesSong Zheng, Jessica Wohlfahrt, Ian Cohen and Yana Cen14. Site-directed labeling of β-arrestin with monobromobimane for measuring their interaction with G protein-coupled receptorsAshish Srivastava, Mithu Baidya, Hemlata Dwivedi-Agnihotri and Arun K. Shukla15. Reversible biotinylation of purified proteins for measuring protein-protein interactionsHemlata Dwivedi-Agnihotri, Ashish Srivastava and Arun K. Shukla