Laboratory Fish in Biomedical Research

Livia D’Angelo is veterinarian by background, she is researcher at University of Naples. Her research activities are mainly focused on the neurobiology of two freshwater fish, zebrafish and the turquoise killifish. She is also deeply involved in the laboratory animal sciences field, with respect to husbandry and management of aquatic research organisms. Paolo de Girolamo is a veterinarian by background, he is full Professor at University of Naples, and he is in charge of Chief of Veterinary Officer and Ethical Animal Care and Use Committee. His research interests have been since long addressed to the neurobiology and gut-brain axis interplay in teleostean fish (goldfish, zebrafish, turquoise killifish etc.).

• Section 1: Zebrafish (Danio rerio)1. Biology and research applicationsBrautigam Lars and Filipek-Go´rniok Beata2. Housing and maintenance of zebrafish, new technologies in laboratory aquatic systems and considerations for facility designBruce Newell and Marco Brocca3. Breeding and larviculture of zebrafish (Danio rerio)Benjamin Tsang and Robert Gerlai4. Health monitoring, disease, and clinical pathologyJean-Philippe Mocho and Nuno Pereira5. The welfare of zebrafishPaul Georg Schroeder6. Analgesia, anesthesia, and euthanasia in zebrafishAlmut Kohler and Ana M. Valentim7. Transgenesis, mutagenesis, knockdown, and genetic colony managementClaire Allen, Francesco Argenton, Fredericus J. Van Eeden and Natascia Tiso8. Sperm cryopreservation, in vitro fertilization, and embryo freezingKanav Khosla, John Bischof and Zolta´n M. VargaSection 2: Other small freshwater fish9. Medaka as a model teleost: characteristics and approaches of genetic modificationTokiro Ishikawa, Yu Murakami, Chika Fujimori, Masato Kinoshita, Kiyoshi Naruse and Shinji Kanda10. Integrated analyses using medaka as a powerful model animal toward understanding various aspects of reproductive regulationChie Umatani, Mikoto Nakajo, Daichi Kayo, Yoshitaka Oka and Shinji Kanda11. The African turquoise killifish (Nothobranchius furzeri): biology and research applicationsGwendoline Astre, Eitan Moses and Itamar Harel12. Challenges in keeping annual killifish Martin Reichard, Radim Blazek, Iva Dykova, Jakub Zak and Matej Polacik13. Mexican tetra (Astyanax mexicanus): biology, husbandry, and experimental protocolsDiana P. Baumann and Andrew Ingalls14. The housing, care, and use of a laboratory three-spined stickleback colonyEllen Blaker, Marion Sebire, Tim Ellis and Ioanna Katsiadaki15. Goldfish (Carassius auratus): biology, husbandry, and research applicationsAyelen M. Blanco and Suraj Unniappan16. Danionella translucida, a tankful of new opportunitiesGokul Rajan, Karine Duroure and Filippo Del BeneSection 3: Databases17. Fish inventory databasesJana Oltova