Current Research in Protein Chemistry

Current Research in Protein Chemistry: Techniques, Structure, and Function focuses on the techniques and methods used for determining the structure and function of proteins. Topics covered range from protein folding and stability to catalysis by chimeric proteins, amino acid and peptide analysis, applications of mass spectrometry to peptide and protein analysis, and protein sequencing. This book is divided into six sections encompassing 55 chapters. The first chapter describes a novel method for protein hydrolysis by means of microwave irradiation that uses Teflon-Pyrex tubes. This is followed by a discussion of the application of high performance capillary electrophoresis to the analysis of amino acids. The sections that follow focus on mass spectrometric methods, protein sequencing, and capillary electrophoresis as well as protein stability, chimeric proteins and enzyme modifications, and protein structure prediction. The crystal structure of human interleukin-1alpha, the acid-denatured states of proteins, solubility of recombinant proteins expressed in Escherichia coli, and catalysis by chimeric proteins are considered. The reader is also introduced to peptide mapping and internal sequencing of proteins from acrylamide gels, new approaches to covalent sequence analysis, alkaline denaturation of hemoglobin, and measurements of disulfide bond stabilities in protein folding intermediates. Students and researchers interested in protein chemistry will find this book extremely helpful.