Dr. Meleady received her degree in Biotechnology from the Dublin City University and PhD from the National Cell and Tissue Culture Centre. She is currently Senior Research Scientist, Programme Leader, Proteomics Core Facility, and Associate Director National Institute for Cell Biology.

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• Section I. Introduction1. Book introduction2. MS Proteomic OverviewSection II. Sample Preparation3. Sample preparation from mammalian cell lines4. Sample preparation for proteomics and MS from clinical tissue5. Sample preparation for proteomics and MS from biological fluids, e.g. serum/plasma from patient blood6. Sample preparation for proteomics and MS from bacterial and fungal samples7. Sample preparation for proteomics and MS from urine samples including exosome isolation8. Sample preparation for proteomics and MS from animal samples9. Subcellular fractionation methods for proteomic analysisSection III. Protein Digestion10. In gel digestion for mass spectrometry11. In solution digestion for mass spectrometrySection IV. MS Proteomic Analysis Protocols12. Protocols for Label-free LC-MS/MS proteomic analysis13. Protocols for TMT labelled LC-MS/MS analysis14. Phosphorylation enrichment for phosphoproteomic analysis15. Ubiquitination enrichment for ubiquitinome PTM analysis16. Glycosylation enrichment and analysis17. LC-MS for protein identification and PTM analysis (e.g. methylation)

Dr. Meleady received her degree in Biotechnology from the Dublin City University and PhD from the National Cell and Tissue Culture Centre. She is currently Senior Research Scientist, Programme Leader, Proteomics Core Facility, and Associate Director National Institute for Cell Biology.

Select Guide Rating

• Section I. Introduction1. Book introduction2. MS Proteomic OverviewSection II. Sample Preparation3. Sample preparation from mammalian cell lines4. Sample preparation for proteomics and MS from clinical tissue5. Sample preparation for proteomics and MS from biological fluids, e.g. serum/plasma from patient blood6. Sample preparation for proteomics and MS from bacterial and fungal samples7. Sample preparation for proteomics and MS from urine samples including exosome isolation8. Sample preparation for proteomics and MS from animal samples9. Subcellular fractionation methods for proteomic analysisSection III. Protein Digestion10. In gel digestion for mass spectrometry11. In solution digestion for mass spectrometrySection IV. MS Proteomic Analysis Protocols12. Protocols for Label-free LC-MS/MS proteomic analysis13. Protocols for TMT labelled LC-MS/MS analysis14. Phosphorylation enrichment for phosphoproteomic analysis15. Ubiquitination enrichment for ubiquitinome PTM analysis16. Glycosylation enrichment and analysis17. LC-MS for protein identification and PTM analysis (e.g. methylation)

Dr. Meleady received her degree in Biotechnology from the Dublin City University and PhD from the National Cell and Tissue Culture Centre. She is currently Senior Research Scientist, Programme Leader, Proteomics Core Facility, and Associate Director National Institute for Cell Biology.

Select Guide Rating

• Section I. Introduction1. Book introduction2. MS Proteomic OverviewSection II. Sample Preparation3. Sample preparation from mammalian cell lines4. Sample preparation for proteomics and MS from clinical tissue5. Sample preparation for proteomics and MS from biological fluids, e.g. serum/plasma from patient blood6. Sample preparation for proteomics and MS from bacterial and fungal samples7. Sample preparation for proteomics and MS from urine samples including exosome isolation8. Sample preparation for proteomics and MS from animal samples9. Subcellular fractionation methods for proteomic analysisSection III. Protein Digestion10. In gel digestion for mass spectrometry11. In solution digestion for mass spectrometrySection IV. MS Proteomic Analysis Protocols12. Protocols for Label-free LC-MS/MS proteomic analysis13. Protocols for TMT labelled LC-MS/MS analysis14. Phosphorylation enrichment for phosphoproteomic analysis15. Ubiquitination enrichment for ubiquitinome PTM analysis16. Glycosylation enrichment and analysis17. LC-MS for protein identification and PTM analysis (e.g. methylation)