Proteomics Mass Spectrometry Methods

Sample Preparation, Protein Digestion, and Research Protocols

Häftad, Engelska, 2024

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Beskrivning

Proteomics Mass Spectrometry Methods: Sample Preparation, Protein Digestion, and Research Protocols shares best practices collected across key laboratories and core facilities, taking the reader through key tactics for executing the most usual mass spectrometry experiments. Sections review research making use of MS proteomics experiments, focus on critical sample preparation, cover mammalian cell lines and samples from clinical tissue and biological fluids, discuss subcellular fractionation, provide methods for protein digestion both for in gel and in solution, and delve into key MS proteomics analysis protocols, including label-free LC-MS, TMT and iTRAQ labelled LC-MS, phosphorylation enrichment, ubiquination enrichment, and more. This book is the perfect lab manual for research teams or for use as a new staff training material. Core facility managers may also find it useful for sharing best practices with their staff and researchers.

Explores the most common questions new researchers have
Guides readers to properly design the workflow for successful integration of mass spectrometry into protein biochemical analyses
Provides examples of sample preparation for a number of different materials, mammalian cells, and others

Produktinformation

Utgivningsdatum:2024-02-13
Mått:191 x 235 x 19 mm
Vikt:710 g
Format:Häftad
Språk:Engelska
Antal sidor:240
Förlag:Elsevier Science
ISBN:9780323903950

Utforska kategorier

Cellbiologi inom Naturvetenskap och teknik

Mer om författaren

Dr. Meleady received her degree in Biotechnology from the Dublin City University and PhD from the National Cell and Tissue Culture Centre. She is currently Senior Research Scientist, Programme Leader, Proteomics Core Facility, and Associate Director National Institute for Cell Biology.

Innehållsförteckning

Section I. Introduction1. Book introduction2. MS Proteomic OverviewSection II. Sample Preparation3. Sample preparation from mammalian cell lines4. Sample preparation for proteomics and MS from clinical tissue5. Sample preparation for proteomics and MS from biological fluids, e.g. serum/plasma from patient blood6. Sample preparation for proteomics and MS from bacterial and fungal samples7. Sample preparation for proteomics and MS from urine samples including exosome isolation8. Sample preparation for proteomics and MS from animal samples9. Subcellular fractionation methods for proteomic analysisSection III. Protein Digestion10. In gel digestion for mass spectrometry11. In solution digestion for mass spectrometrySection IV. MS Proteomic Analysis Protocols12. Protocols for Label-free LC-MS/MS proteomic analysis13. Protocols for TMT labelled LC-MS/MS analysis14. Phosphorylation enrichment for phosphoproteomic analysis15. Ubiquitination enrichment for ubiquitinome PTM analysis16. Glycosylation enrichment and analysis17. LC-MS for protein identification and PTM analysis (e.g. methylation)