Methods in Cell Wall Cytochemistry

Krishnamurthy, K V

• PrefaceCell Wall-Morphology & Chemical CompositionCarbohydratesCellulose, Callose and other 1, 3-b-glucans, Chitins, Pectins, Hemicelluloses, Alginic Acid, Sulphated PolysaccharidesGlycoproteinsStructural Proteins-Lectins, Arabinogalacton Rich Proteins (AGP's), Hydroxy Proline Rich Proteins, Proline Rich Proteins, Glycine Rich Proteins, Threonine Rich Proteins, Thionins, Pistil Specific Extensin-Like ProteinsEnzymes-Peroxidase, Polyamineoxidase, Phosphatase, Protease, Nitrate Reductase, Malate Dehydrogenase, Proline Hydroxylase, Glycosylhydrolases and Transferases, Non-Specific Esterases, and RibonucleusesLipids and Related Substances-Cutin, Suberin, Waxes, Sporopollenin, Pollenkitt, and TryphineLignin and Other Phenolic Substances-Lignins, Phenolic Acids and AmidesMineral Substances-Silica, Calcium, and BoronLight Microscopic CytochemistryIntroductionTissue Preparation-Use of Fresh Living Tissue, Use of Frozen Tissue, Freeze Substitution and Drying, Chemical Fixation, Dehydration and Clearing, Infiltration and Embedding, Sectioning of MaterialsStains and Staining-Introduction, Control Procedures, Incubation of MaterialsMounting of Stained MaterialsProcedures in Tissue Preparation for Light Microscopic Cytochemistry-Freezing, Freeze Substitution and Drying Methods, Preparation of Fixatives, Dehydration and Clearing ProceduresProcedures for the Cytochemical Localizations of Different Cell Wall SubstancesCarbohydrates-Insoluble Polysaccharides, Cellulose, Chitin, Callose, Sulphated and Carboxylated polysaccharidesProteins-Non-Specific Cell Wall Proteins, Enzymic ProteinsLigninsSporopolleninsCutins & SuberinMineralsFluorescent Microscopic CytochemistryIntroductionFluorescent Cytochemical MethodsCell Wall CarbohydratesCell Wall ProteinsLigninFerulic AcidSporopolleninCutin and SuberinTransmission Electron Microscopic CytochemistryIntroducti