Flow Cytometry Applications in Cell Culture

Mohamed Al-Rubeai, A. Nicholas Emery

• Monitoring the proliferative capacity of cultured animal cells by cell cycle analysis; use of flow cytometry for monitoring antibody productivity and isolating high-secreting hybridoma cells; antibody secretion assays using gel microdrops and flow cytometry; high resolution cell cycle analysis of cell cycle-regulated gene expression; stability of monoclonal antibody production in hybridoma cell culture; flow cytometric studies of osmotically stressed and sodium butyrate-treated hybridoma cells; flow cytometric analysis of cells obtained from human bone marrow cultures; measurement of intracellular pH during the cultivation of hybridoma cells in batch and continuous cultures; the relationship between intracellular pH and cell cycle in cultured animal cells using SNARF-1 indicator; assessment of cell viability in mammalian cell lines; analysis of apoptosis by flow cytometry; monitoring baculovirus infection and protein expression in insect cells; use of protein distribution to analyze budding yeast population structure and cell cycle progression; cell cycle analysis of microorganisms; bacterial characterization by flow cytometry; assessment of viability of bacteria by flow cytometry; advances in the flow cytometric characterization of plant cells and tissues.