Method Development in Analytical HPLC

Dr. Serban C. Moldoveanu held several teaching positions at the University of Bucharest, Romania, until 1983, when he immigrated to the United States. There, he continued teaching at the university level for several years before focusing primarily on industrial research. He served as Senior Principal Scientist at R.J. Reynolds Tobacco Company and retired at the end of 2023. His research has centered on various aspects of chromatography, including method development for the analysis of natural products using GC/MS, HPLC, and LC/MS/MS. He has also investigated the pyrolysis of a variety of polymers and organic compounds. Dr. Moldoveanu has published more than 150 articles in peer-reviewed journals, 8 books with Elsevier, approximately 22 patents/patent applications, and several book chapters. He was honored with the Tobacco Science Research Conference Lifetime Achievement Award in 2012 and currently serves as an editor for three chemistry journals.Victor David was a Professor and Head of the Department of Analytical Chemistry, University of Bucharest, Romania. Recently retired, he remains Emeritus Professor at the same Department. His main field of research is separation science (theory and applications in various scientific domains). He is the author of more than 200 publications, including over 150 scientific papers in peer reviewed journals and 25 books and chapters. He is member of editorial board of Biomedical Chromatography, Molecules, Journal of Essential Oil-Bearing Plants and Revue Roumaine de Chimie.

• 1. Introductory concepts related to a chemical analysis2. Retention and elution processes in HPLC3. Instrumentation in HPLC4. Performance and utilization of HPLC separation and detection5. Samples for HPLC analysis6. Sample preparation for an HPLC analysis7. Chromatographic columns in HPLC8. Mobile phase and the role of solvents in HPLC9. Development of an HPLC analytical method10. Selection of the best options in developing an HPLC analytical method11. Method and data validation in HPLC analysis