Protein Termini Part B

After completing studies for the A.B., A.M., and Ph.D. degrees in chemistry at Harvard University, David W. Christianson joined the faculty of the University of Pennsylvania, where he is currently the Roy and Diana Vagelos Professor in Chemistry and Chemical Biology. At Penn, Christianson’s research focuses on the structural and chemical biology of the zinc-dependent histone deacetylases as well as enzymes of terpene biosynthesis. His research accomplishments have been recognized by several awards, including the Pfizer Award in Enzyme Chemistry and the Repligen Award in Chemistry of Biological Processes from the American Chemical Society, a Guggenheim Fellowship, and the Elizabeth S. and Richard M. Cashin Fellowship from the Radcliffe Institute for Advanced Study at Harvard University. Christianson is also a dedicated classroom teacher, and his accomplishments in this regard have been recognized by the Lindback Award for Distinguished Teaching at Penn and a Rhodes Trust Inspirational Educator Award from Oxford University. Christianson has also held visiting professorships in the Department of Biochemistry at Cambridge University and the Department of Chemistry and Chemical Biology at Harvard University. Christianson has served with Prof. Anna Pyle as Co-Editor-in-Chief of Methods in Enzymology since 2015.Dr. Karen N. Allen works at the Department of Chemistry of the Boston University, the Metcalf Center for Science and Engineering Professor Thomas Arnesen received his Ph.D. in molecular biology from the University of Bergen, Norway in 2006. After postdoctoral work at Haukeland University Hospital and University of Rochester Medical Center, he established his own lab at the University of Bergen in 2010. His main interest has been protein N-terminal acetylation and the responsible enzymes, the N-terminal acetyltransferases (NATs). Using Saccharomyces cerevisiae and human cell models combined with in vitro approaches his lab and collaborators have i) identified and defined the presumed complete cytosolic human NAT-machinery including NATs acting post-translationally, ii) quantitatively analysed the N-terminal acetylomes of yeast and human cells, iii) developed novel assays for NAT-profiling, iv) gained mechanistic insights of the molecular and cellular effects of N-terminal acetylation, v) contributed to the understanding of the physiological and clinical importance of NATs by revealing the links between NatA and cancer cell survival and drug sensitisation, and lately by defining genetic disorders caused by pathogenic NAT variants. The Arnesen lab also contributed to solving the first NAT-structures and developing the first potent NAT-inhibitors. With Fred Sherman and Bogdan Polevoda, Arnesen introduced the NAA (N-alpha acetyltransferase) nomenclature of the N-terminal acetyltransferase genes and proteins, and he acts as the specialist advisor for the HUGO Gene Nomenclature Committee for these genes. Arnesen is one of the founders and council members of the International Society of Protein Termini (ISPT). He has organized several symposia on N-terminal acetylation, and in 2022 he was the head organizer of the EMBO Workshop ‘Protein Termini – From mechanism to biological impact’ in Bergen, Norway. Arnesen has co-authored more than 100 peer-reviewed publications. Today he is head of the Translational Cell Signaling and Metabolism group at the Dept. of Biomedicine, UiB, supported by the Research Council of Norway and ERC. Here his team continues the basic and translational research to understand the impact of protein N-terminal modifications.

• 1. Proteome analysis of puromycin-labeled nascent polypeptidesKoshi Imami2. HUNTER-DIA: An updated protocol for enrichment and mass spectrometry-based identification of protein N-terminiPitter F. Huesgen3. TERMINER - Bioinformatic detection and annotation of proteolytic protein termini in shotgun proteomics dataOliver Schilling4. Degronopedia: A practical guide to identifying and targeting protein degronsWojciech Pokrzywa5. Quantitative Insights into Protein Turnover and Ubiquitination with HiBiT and NanoBRETWojciech Pokrzywa6. Development of a flow cytometric method to evaluate the impact of N-terminal sequences on protein stabilityAditya M. Kunjapur7. Generation and characterization of engineered N-degrons of the N-degron pathway using the ubiquitin-reference techniqueChang Hoon Ji8. Characterization of the autophagic N-degron pathway and monitoring its chemical modulation for therapeutic developmentYong Tae Kwon9. Characterization of the E3 ligase KCMF1 as a ZZ/N-recognin of the autophagic Arg/N-degron pathwayChang Hoon Ji10. Identification of Ac/N-degron-recognition domain within the MARCHF6 E3 ubiquitin ligaseCheol-Sang Hwang11. Affinity purification-mass spectrometry to identify nuclear protein interactions of N-terminal acetyltransferase NAA40Antonis Kirmizis12. TurboID technique for proximity labelling of interacting proteinsGreta Jarck13. Chemical proteomic approaches to investigate S-prenylationEdward W. Tate14. Quantitative analysis of C-terminal prenylated protein levels using tandem mass taggingMark Distefano15. Optimizing purification and FP-based binding assays for the E3 ligase FEM1CRong Huang