SLOBODAN SASIC works in the Pharmaceutical Sciences Department at Pfizer Global Research and Development. He is a well-published and recognized authority on Raman spectroscopy for pharmaceutical analysis. Dr. Sasic edited Pharmaceutical Applications of Raman Spectroscopy (Wiley). YUKIHIRO OZAKI is a Professor in the Department of Chemistry at Kwansei Gakuin University. He is also a coeditor of the books Near-Infrared Spectroscopy in Food Science and Technology, Near-Infrared Spectroscopy: Principles, Instruments, Applications, and Two-Dimensional Correlation Spectroscopy: Applications in Vibrational and Optical Spectroscopy, all published by Wiley.

“The book is a valuable resource for everyone working in vibrational spectroscopic imaging and will inspire researchers to exploit the exciting capabilities of these powerful and multifaceted techniques.”  (Anal Bioanal Chem, 2011)

• Preface viiContributors ix1. Spectroscopic Theory for Chemical Imaging 1M. J. Pelletier and C. C. PelletierPart I Hardware 212. Raman Imaging Instrumentation 23Matthew P. Nelson and Patrick J. Treado3. FT-IR Imaging Hardware 55J. Sellors, R. A. Hoult, R. A. Crocombe, and N. A. Wright4. Technologies and Practical Considerations for Implementing Near-Infrared Chemical Imaging 75E. Neil Lewis and Linda H. Kidder5. Data Analysis and Chemometrics for Hyperspectral Imaging 93Paul Geladi, Hans Grahn, and Marena ManleyPart II Biomedical Applications 1096. Biomedical Applications of Raman Imaging 111Michael D. Morris and Gurjit S. Mandair7. Skin Pharmacology and Cosmetic Science Applications of IR Spectroscopy, Microscopy, and Imaging 133Richard Mendelsohn, Carol R. Flach, David J. Moore, and Laurence Senak8. Near-Infrared In Vivo Spectroscopic Imaging: Biomedical Research and Clinical Applications 149R. Anthony Shaw, Valery V. Kupriyanov, Olga Jilkina, and Michael G. SowaPart III Pharmaceutical Applications 1679. Pharmaceutical Applications of Raman Chemical Imaging 169Slobodan Šašić and Lin Zhang10. Applications of FTIR Spectroscopic Imaging in Pharmaceutical Science 185Sergei G. Kazarian and Patrick S. Wray11. NIR Imaging Applications in the Pharmaceutical Industry 205Ad Gerich, Janie Dubois, and Linda H. KidderPart IV Applications in Food Research 22712. Raman and Infrared Imaging of Foods 229Nils Kristian Afseth and Ulrike Böker13. Near-Infrared Hyperspectral Imaging in Food Research 243Paul Geladi and Marena ManleyPart V Applications in Polymer Research 26114. Vibrational Spectroscopy Imaging in Polymers 263Harumi Sato, Yukihiro Ozaki, Jianhui Jiang, Ru-Qin Yu, and Hideyuki ShinzawaPart VI Special Methods 28315. Surface-Enhanced Raman Scattering Imaging: Application and Experimental Approach by Far-Field with Conventional Setup 285Yasutaka Kitahama, Mohammad Kamal Hossain, Yukihiro Ozaki, Tamitake Itoh, Athiyanathil Sujith, and Xiaoxia Han16. Linear and Nonlinear Raman Microspectroscopy: From a Molecule to Single Living Cells 301Hideaki Kano, Yu-San Huang, Yasuaki Naito, Rintaro Shimada, and Hiro-o HamaguchiIndex 313

SLOBODAN SASIC works in the Pharmaceutical Sciences Department at Pfizer Global Research and Development. He is a well-published and recognized authority on Raman spectroscopy for pharmaceutical analysis. Dr. Sasic edited Pharmaceutical Applications of Raman Spectroscopy (Wiley). YUKIHIRO OZAKI is a Professor in the Department of Chemistry at Kwansei Gakuin University. He is also a coeditor of the books Near-Infrared Spectroscopy in Food Science and Technology, Near-Infrared Spectroscopy: Principles, Instruments, Applications, and Two-Dimensional Correlation Spectroscopy: Applications in Vibrational and Optical Spectroscopy, all published by Wiley.

“The book is a valuable resource for everyone working in vibrational spectroscopic imaging and will inspire researchers to exploit the exciting capabilities of these powerful and multifaceted techniques.”  (Anal Bioanal Chem, 2011)

• Preface viiContributors ix1. Spectroscopic Theory for Chemical Imaging 1M. J. Pelletier and C. C. PelletierPart I Hardware 212. Raman Imaging Instrumentation 23Matthew P. Nelson and Patrick J. Treado3. FT-IR Imaging Hardware 55J. Sellors, R. A. Hoult, R. A. Crocombe, and N. A. Wright4. Technologies and Practical Considerations for Implementing Near-Infrared Chemical Imaging 75E. Neil Lewis and Linda H. Kidder5. Data Analysis and Chemometrics for Hyperspectral Imaging 93Paul Geladi, Hans Grahn, and Marena ManleyPart II Biomedical Applications 1096. Biomedical Applications of Raman Imaging 111Michael D. Morris and Gurjit S. Mandair7. Skin Pharmacology and Cosmetic Science Applications of IR Spectroscopy, Microscopy, and Imaging 133Richard Mendelsohn, Carol R. Flach, David J. Moore, and Laurence Senak8. Near-Infrared In Vivo Spectroscopic Imaging: Biomedical Research and Clinical Applications 149R. Anthony Shaw, Valery V. Kupriyanov, Olga Jilkina, and Michael G. SowaPart III Pharmaceutical Applications 1679. Pharmaceutical Applications of Raman Chemical Imaging 169Slobodan Šašić and Lin Zhang10. Applications of FTIR Spectroscopic Imaging in Pharmaceutical Science 185Sergei G. Kazarian and Patrick S. Wray11. NIR Imaging Applications in the Pharmaceutical Industry 205Ad Gerich, Janie Dubois, and Linda H. KidderPart IV Applications in Food Research 22712. Raman and Infrared Imaging of Foods 229Nils Kristian Afseth and Ulrike Böker13. Near-Infrared Hyperspectral Imaging in Food Research 243Paul Geladi and Marena ManleyPart V Applications in Polymer Research 26114. Vibrational Spectroscopy Imaging in Polymers 263Harumi Sato, Yukihiro Ozaki, Jianhui Jiang, Ru-Qin Yu, and Hideyuki ShinzawaPart VI Special Methods 28315. Surface-Enhanced Raman Scattering Imaging: Application and Experimental Approach by Far-Field with Conventional Setup 285Yasutaka Kitahama, Mohammad Kamal Hossain, Yukihiro Ozaki, Tamitake Itoh, Athiyanathil Sujith, and Xiaoxia Han16. Linear and Nonlinear Raman Microspectroscopy: From a Molecule to Single Living Cells 301Hideaki Kano, Yu-San Huang, Yasuaki Naito, Rintaro Shimada, and Hiro-o HamaguchiIndex 313

SLOBODAN SASIC works in the Pharmaceutical Sciences Department at Pfizer Global Research and Development. He is a well-published and recognized authority on Raman spectroscopy for pharmaceutical analysis. Dr. Sasic edited Pharmaceutical Applications of Raman Spectroscopy (Wiley). YUKIHIRO OZAKI is a Professor in the Department of Chemistry at Kwansei Gakuin University. He is also a coeditor of the books Near-Infrared Spectroscopy in Food Science and Technology, Near-Infrared Spectroscopy: Principles, Instruments, Applications, and Two-Dimensional Correlation Spectroscopy: Applications in Vibrational and Optical Spectroscopy, all published by Wiley.

“The book is a valuable resource for everyone working in vibrational spectroscopic imaging and will inspire researchers to exploit the exciting capabilities of these powerful and multifaceted techniques.”  (Anal Bioanal Chem, 2011)

• Preface viiContributors ix1. Spectroscopic Theory for Chemical Imaging 1M. J. Pelletier and C. C. PelletierPart I Hardware 212. Raman Imaging Instrumentation 23Matthew P. Nelson and Patrick J. Treado3. FT-IR Imaging Hardware 55J. Sellors, R. A. Hoult, R. A. Crocombe, and N. A. Wright4. Technologies and Practical Considerations for Implementing Near-Infrared Chemical Imaging 75E. Neil Lewis and Linda H. Kidder5. Data Analysis and Chemometrics for Hyperspectral Imaging 93Paul Geladi, Hans Grahn, and Marena ManleyPart II Biomedical Applications 1096. Biomedical Applications of Raman Imaging 111Michael D. Morris and Gurjit S. Mandair7. Skin Pharmacology and Cosmetic Science Applications of IR Spectroscopy, Microscopy, and Imaging 133Richard Mendelsohn, Carol R. Flach, David J. Moore, and Laurence Senak8. Near-Infrared In Vivo Spectroscopic Imaging: Biomedical Research and Clinical Applications 149R. Anthony Shaw, Valery V. Kupriyanov, Olga Jilkina, and Michael G. SowaPart III Pharmaceutical Applications 1679. Pharmaceutical Applications of Raman Chemical Imaging 169Slobodan Šašić and Lin Zhang10. Applications of FTIR Spectroscopic Imaging in Pharmaceutical Science 185Sergei G. Kazarian and Patrick S. Wray11. NIR Imaging Applications in the Pharmaceutical Industry 205Ad Gerich, Janie Dubois, and Linda H. KidderPart IV Applications in Food Research 22712. Raman and Infrared Imaging of Foods 229Nils Kristian Afseth and Ulrike Böker13. Near-Infrared Hyperspectral Imaging in Food Research 243Paul Geladi and Marena ManleyPart V Applications in Polymer Research 26114. Vibrational Spectroscopy Imaging in Polymers 263Harumi Sato, Yukihiro Ozaki, Jianhui Jiang, Ru-Qin Yu, and Hideyuki ShinzawaPart VI Special Methods 28315. Surface-Enhanced Raman Scattering Imaging: Application and Experimental Approach by Far-Field with Conventional Setup 285Yasutaka Kitahama, Mohammad Kamal Hossain, Yukihiro Ozaki, Tamitake Itoh, Athiyanathil Sujith, and Xiaoxia Han16. Linear and Nonlinear Raman Microspectroscopy: From a Molecule to Single Living Cells 301Hideaki Kano, Yu-San Huang, Yasuaki Naito, Rintaro Shimada, and Hiro-o HamaguchiIndex 313