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      1. Naturvetenskap och teknik
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      Redox Biocatalysis

      Fundamentals and Applications

      AvDaniela Gamenara,Gustavo Seoane

      Inbunden, Engelska, 2012

      1 935 kr

      Beställningsvara. Skickas inom 5-8 vardagar. Fri frakt över 249 kr.

      Beskrivning

      Paves the way for new industrial applications using redox biocatalysisIncreasingly, researchers rely on the use of enzymes to perform redox processes as they search for novel industrial synthetic routes. In order to support and advance their investigations, this book provides a comprehensive and current overview of the use of redox enzymes and enzyme-mediated oxidative processes, with an emphasis on the role of redox enzymes in chemical transformations. The authors examine the full range of topics in the field, from basic principles to new and emerging research and applications. Moreover, they explore everything from laboratory-scale procedures to industrial manufacturing.Redox Biocatalysis begins with a discussion of the biochemical features of redox enzymes as well as cofactors and cofactor regeneration methods. Next, the authors present a variety of topics and materials to the research and development of full-scale industrial applications, including: Biocatalytic applications of redox enzymes such as dehydrogenases, oxygenases, oxidases, and peroxidasesEnzyme-mediated oxidative processes based on biocatalytic promiscuityAll the steps from enzyme discovery to robust industrial processes, including directed evolution, high-throughput screening, and medium engineeringCase studies tracing the development of industrial applications using biocatalytic redox reactionsEach chapter ends with concluding remarks, underscoring the key scientific principles and processes. Extensive references serve as a gateway to the growing body of research in the field.Researchers in both academia and industry will find this book an indispensable reference for redox biotransformations, guiding them from underlying core principles to new discoveries and emerging industrial applications.

      Produktinformation

      • Utgivningsdatum:2012-11-16
      • Mått:165 x 241 x 29 mm
      • Vikt:903 g
      • Format:Inbunden
      • Språk:Engelska
      • Antal sidor:552
      • Förlag:John Wiley & Sons Inc
      • ISBN:9780470874202

      Utforska kategorier

      • Tillverkningsteknik inom Naturvetenskap och teknik

      Mer om författaren

      Daniela Gamenara, PhD, is Assistant Professor in the Organic Chemistry Department of the Faculty of Chemistry of Universidad de la República, Uruguay. After two short postdoctoral internships, her current scientific interest is the development of enzymatic methodologies for the synthesis of high added-value compounds and natural products as well, together with the use of new trends in organocatalysis for the same purposes.Gustavo A. Seoane, PhD, is Full Professor and Head of the Organic Chemistry Department of the Faculty of Chemistry of Universidad de la República, Uruguay. His main scientific interest is the use of green procedures for the synthesis of bioactive natural products and analogs, in particular, intensive use of biotransformations to prepare polyoxygenated targets. He works actively to promote the development of biocatalysis in South America.Patricia Saenz-Méndez, PhD, is Assistant Professor of Physical Organic Chemistry in the Faculty of Chemistry of Universidad de la República, Uruguay. She was previously appointed as postdoctoral fellow at the Örebro University, Sweden, and the National University of Ireland, Galway. Her work is devoted to the experimental and in silico development of biotechnological and organocatalytic tools for the preparation of high added-value chemicals and natural products.Pablo Domínguez de María, PhD, is Group Leader at the Institute for Technical and Macromolecular Chemistry (ITMC), RWTH Aachen University, Germany. He was previously affiliated with AkzoNobel BV in the Netherlands and Degussa AG in Germany. Dr. Domínguez de María has also worked as a technical freelance advisor on emerging technologies (e.g., catalysis) applied to sustainable chemistry (www.sustainablemomentum.net). He was awarded the Young Scientist Prize by the Iberoamerican Academy of Pharmacy.

      Innehållsförteckning

      • PREFACE ix1. Enzymes Involved in Redox Reactions: Natural Sources and Mechanistic Overview 11.1 Motivation: Green Chemistry and Biocatalysis 11.2 Sources of Biocatalysts 21.2.1 Plants and Animals as Sources of Redox Biocatalysts 31.2.2 Wild-Type Microorganisms 71.2.2.1 Yeasts 71.2.2.2 Fungi 81.2.2.3 Bacteria 81.2.3 Metagenomic Assessments 91.3 Overview of Redox Enzymes 101.3.1 Dehydrogenases 131.3.1.1 Zn-Dependent Dehydrogenases 141.3.1.2 Flavin-Dependent Dehydrogenases 151.3.1.3 Pterin-Dependent Dehydrogenases 161.3.1.4 Quinoprotein Dehydrogenases 171.3.1.5 Dehydrogenases without Prosthetic Group 181.3.2 Oxygenases 191.3.2.1 Monooxygenases 201.3.2.2 Dioxygenases 381.3.3 Oxidases 501.3.3.1 Iron-Containing Oxidases 501.3.3.2 Copper-Containing Oxidases 511.3.3.3 Flavin-Dependent Oxidases 561.3.4 Peroxidases 611.4 Concluding Remarks 64References 642. Natural Cofactors and Their Regeneration Strategies 862.1 Types of Natural Cofactors—Mechanisms 862.2 Cofactor Regeneration 882.2.1 Enzymatic Regeneration of Reduced Cofactors 882.2.1.1 Substrate-Assisted Method 882.2.1.2 Enzyme-Assisted Method 892.2.2 Enzymatic Regeneration of Oxidized Cofactors 922.2.3 Chemical Regeneration of Cofactors 942.2.4 Electrochemical Regeneration of Cofactors 952.2.5 Photochemical Regeneration of Cofactors 962.3 Concluding Remarks 97References 983. Reactions Involving Dehydrogenases 1013.1 General Considerations 1013.2 Reduction of Carbonyl Groups 1053.2.1 Reduction of Aliphatic and Aromatic Ketones 1063.2.2 Reduction of α- and β-keto Esters and Derivatives 1193.2.3 Reduction of Diketones 1263.2.4 Reduction of Aldehydes 1283.3 Racemization and Deracemization Reactions 1303.4 Preparation of Amines 1353.5 Reduction of C–C Double Bonds 1423.6 Oxidation Reactions 1523.7 Dehydrogenase-Catalyzed Redox Reactions in Natural Products 1593.8 Concluding Remarks 164References 1654. Reactions Involving Oxygenases 1804.1 Monooxygenase-Catalyzed Reactions 1804.1.1 Hydroxylation of Aliphatic Compounds 1814.1.2 Hydroxylation of Aromatic Compounds 1874.1.3 Baeyer–Villiger Reactions 1894.1.3.1 Classification and Metabolic Role of BVMOs 1924.1.3.2 Isolated Enzymes versus Whole-Cell Systems (Wild-Type and Recombinant Microorganisms) 1944.1.3.3 Substrate Profile of Available Baeyer–Villiger Monooxygenases 1954.1.3.4 Synthetic Applications of BVMOs 2014.1.4 Epoxidation of Alkenes 2404.2 Dioxygenase-Catalyzed Reactions 2514.2.1 Aromatic Dioxygenases 2514.2.1.1 Dihydroxylation of Aromatic Compounds 2514.2.1.2 Other Oxidation Reactions Performed by Aromatic Dioxygenases 2744.2.2 Miscellaneous Dioxygenases 2794.2.2.1 Lipoxygenase 2794.3 Concluding Remarks 285References 2865. Reactions Involving Oxidases and Peroxidases 3035.1 Oxidase-Catalyzed Reactions 3045.1.1 Oxidases Acting on C–O Bonds 3045.1.1.1 Galactose Oxidase 3045.1.1.2 Pyranose Oxidase 3085.1.1.3 Alcohol Oxidase 3115.1.1.4 Glucose Oxidase 3135.1.1.5 Glycolate Oxidase 3135.1.2 Laccases and Tyrosinases (Phenol Oxidases) 3155.1.2.1 Laccase 3155.1.2.2 Tyrosinase and Other Polyphenol Oxidases 3525.1.3 Oxidases Acting on C–N Bonds 3615.1.3.1 d-Amino Acid Oxidase 3615.1.3.2 l-Amino Acid Oxidase 3685.1.3.3 Monoamine Oxidase 3685.1.3.4 Copper Amine Oxidases 3715.1.4 Miscellaneous 3715.1.4.1 Cholesterol Oxidase 3725.1.4.2 Vanillyl Alcohol Oxidase 3735.1.4.3 Alditol Oxidase 3735.2 Peroxidase-Catalyzed Reactions 3755.2.1 Peroxidase Mediated Transformations 3795.2.1.1 Oxidative Dehydrogenation (2 RH + H2O2 → 2 R• + 2 H2O→R-R) 3795.2.1.2 Oxidative Halogenation (RH + H2O2 +X− + H+→RX + 2 H2O) 3855.2.1.3 Oxygen-Transfer Reactions (RH + H2O2 → ROH + H2O) 3905.3 Concluding Remarks 403References 4046. Hydrolase-Mediated Oxidations 4336.1 Hydrolase Promiscuity and in situ Peracid Formation. Perhydrolases vs. Hydrolases. Other Promiscuous Hydrolase-Mediated Oxidations 4336.2 Hydrolase-Mediated Bulk Oxidations in Aqueous Media (e.g., Bleaching, Disinfection, etc.) 4366.3 Lipase-Mediated Oxidations: Prileshajev Epoxidations and Baeyer–Villiger Reactions 4396.4 Hydrolase-Mediated Oxidation and Processing of Lignocellulosic Materials 4456.5 Concluding Remarks 448References 4487. Bridging Gaps: From Enzyme Discovery to Bioprocesses 4537.1 Context 4537.2 Enzyme Directed Evolution and High-Throughput-Screening of Biocatalysts 4547.3 Successful Case: Baker’s Yeast Redox Enzymes, Their Cloning, and Separate Overexpression 4677.4 Whole-Cells vs. Isolated Enzymes: Medium Engineering 4737.5 Beyond: Multistep Domino Biocatalytic Processes 4777.6 Concluding Remarks 482References 4838. Industrial Applications of Biocatalytic Redox Reactions: From Academic Curiosities to Robust Processes 4878.1 Motivation: Drivers for Industrial Biocatalytic Processes 4878.2 Key Aspects in Industrial Biocatalytic Processes 4888.3 Industrial Biocatalytic Redox Processes: Free Enzymes 4928.4 Industrial Biocatalytic Redox Processes—Whole-Cells: The “Designer Bug” Concept and Beyond (Metabolic Engineering) 5008.5 Concluding Remarks and Future Perspectives 511References 516INDEX 521
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