Experiments in Plant Tissue Culture

Häftad, Engelska, 1995

783 kr

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Beskrivning

In this third edition the authors have written a guide to the essentials of plant tissue culture. The book takes the reader through a graded series of experimental protocols and provides an introductory review of each topic. There are discussions of aseptic techniques, and nutritional components of media. Subsequent chapters cover such topics as callus formation, organogenesis, xylem cell differentiation, root cultures, cell suspensions, micropropagation by buds, somatic embryogenesis, protoplast culture and fusion, secondary metabolite production, and quantitation of procedures, amongst others. A glossary, commercial sources of supplies, and the formulations of culture media are also included. This new edition has been completely revised and updated. The organization has been improved, and new illustrations added, together with new experiments on such topics as potato callus formation, and embryo culture.

Produktinformation

Utgivningsdatum:1995-01-27
Mått:152 x 228 x 14 mm
Vikt:369 g
Format:Häftad
Språk:Engelska
Antal sidor:276
Upplaga:3
Förlag:Cambridge University Press
Medarbetare:J. Heslop-Harrison
ISBN:9780521478922

Utforska kategorier

Växtböcker inom Djur och Natur
Botanik inom Naturvetenskap och teknik
Cellbiologi inom Naturvetenskap och teknik

Recensioner i media

'This well thought out book does considerably more than is claimed in the title. It not only takes the reader through a graded series of experimental protocols covering the major techniques used in plant tissue culture, it also provides a good introductory review of each topic.' Trends in Biochemical Sciences

Innehållsförteckning

Part I. Background Information: 1. Culture of plant cells, tissues, and organs; 2. Laboratory facilities; 3. Aseptic techniques; 4. Media composition and preparation; Part II. Experimental: Callus and Callus-Derived Systems: 5. Initiation and maintenance of callus; 6. Organogenesis; 7. Cell suspensions; 8. Somatic embryogenesis; Part III. Experimental: Culture of Organs and Organized Systems: 9. Isolated roots; 10. Micropropagation by bud proliferation; 11. Anther and pollen cultures; Part IV. Experimental: Isolated Cells: 12. Transdifferentiation of parenchyma cells to tracheary elements; 13. Isolation and culture of protoplasts; 14. Protoplast fusion and somatic hybridization; Part V. Supplementary Topics: 15. Cryopreservation of germplasm; 16. Production of secondary metabolites; 17. Quantitation of procedures; 18. Formulations of tissue culture media; 19. Commercial sources of supplies; Author index; Subject index.