"...the authors are to be commended for the consistency in format and altruism shown contributors to represent the procedures with minimal self-serving recounting of their use of the method."-The Quarterly Review of Biology

• to Polymerase Chain Reaction.- Methodological Issues in Polymerase Chain Reaction for RNA.- Subtractive Hybridization of cDNA Libraries.- The Use of Differential Display RT-PCR for Identifying Altered Gene Expression in Ischemic Brain Injury.- Analysis of Gene Expression by Multiprobe RNase Protection Assay.- Molecular Probes for PNS Neurotoxicity, Degeneration, and Regeneration.- Mobility-Shift DNA-Binding Assay Using Gel Electrophoresis.- Transfection of Mammalian Cells In Vitro: Use in Analysis of Neuronal Damage.- Detection of Apoptotic Cells in the Nervous System.- Translocation Assays of Protein Kinase C Activation.- Astrocyte and Neuron Coculturing Method.- Viability Assays for Cells In Vitro: The Ethidium/Calcein Assay and the Immunofluorescence Combination Assay.- Measurement of the Nitric Oxide Synthase Activity Using the Citrulline Assay.- Measurement of Intraneuronal Free Calcium Using the Fluorescent Probe Technique.- Measurement of Calcium Buffering by Intracellular Organelles in Brain.- Receptor-Mediated Release of Inositolphosphates in Brain Slices.- The Use of Enzyme-Linked Immunosorbent Assays to Quantitate Proteins in Biological Functions.- Assays for the Analysis of Synaptic Proteins in Neurodegenerative Disorders.- Zymographic Method for the Measurement of Gelatinase Activity in Brain Tissue.- The Measurement of Monoamine Neurotransmitters in Microdialysis Perfusates Using HPLC-ECD.- Biochemical Measurement of Cholinesterase Activity.- Functional Study of Glutamate Receptor Channels in Brain Slices.- Selective Silver Staining for Neural Degeneration.- Immunohistological Markers of Cerebrovascular Integrity.- Electron Probe X-Ray Microanalysis: A Quantitative Electron Microscopy Technique for Measurement of Elements and Water in Nervous Tissue Cells.

"...the authors are to be commended for the consistency in format and altruism shown contributors to represent the procedures with minimal self-serving recounting of their use of the method."-The Quarterly Review of Biology

• to Polymerase Chain Reaction.- Methodological Issues in Polymerase Chain Reaction for RNA.- Subtractive Hybridization of cDNA Libraries.- The Use of Differential Display RT-PCR for Identifying Altered Gene Expression in Ischemic Brain Injury.- Analysis of Gene Expression by Multiprobe RNase Protection Assay.- Molecular Probes for PNS Neurotoxicity, Degeneration, and Regeneration.- Mobility-Shift DNA-Binding Assay Using Gel Electrophoresis.- Transfection of Mammalian Cells In Vitro: Use in Analysis of Neuronal Damage.- Detection of Apoptotic Cells in the Nervous System.- Translocation Assays of Protein Kinase C Activation.- Astrocyte and Neuron Coculturing Method.- Viability Assays for Cells In Vitro: The Ethidium/Calcein Assay and the Immunofluorescence Combination Assay.- Measurement of the Nitric Oxide Synthase Activity Using the Citrulline Assay.- Measurement of Intraneuronal Free Calcium Using the Fluorescent Probe Technique.- Measurement of Calcium Buffering by Intracellular Organelles in Brain.- Receptor-Mediated Release of Inositolphosphates in Brain Slices.- The Use of Enzyme-Linked Immunosorbent Assays to Quantitate Proteins in Biological Functions.- Assays for the Analysis of Synaptic Proteins in Neurodegenerative Disorders.- Zymographic Method for the Measurement of Gelatinase Activity in Brain Tissue.- The Measurement of Monoamine Neurotransmitters in Microdialysis Perfusates Using HPLC-ECD.- Biochemical Measurement of Cholinesterase Activity.- Functional Study of Glutamate Receptor Channels in Brain Slices.- Selective Silver Staining for Neural Degeneration.- Immunohistological Markers of Cerebrovascular Integrity.- Electron Probe X-Ray Microanalysis: A Quantitative Electron Microscopy Technique for Measurement of Elements and Water in Nervous Tissue Cells.

"...the authors are to be commended for the consistency in format and altruism shown contributors to represent the procedures with minimal self-serving recounting of their use of the method."-The Quarterly Review of Biology

• to Polymerase Chain Reaction.- Methodological Issues in Polymerase Chain Reaction for RNA.- Subtractive Hybridization of cDNA Libraries.- The Use of Differential Display RT-PCR for Identifying Altered Gene Expression in Ischemic Brain Injury.- Analysis of Gene Expression by Multiprobe RNase Protection Assay.- Molecular Probes for PNS Neurotoxicity, Degeneration, and Regeneration.- Mobility-Shift DNA-Binding Assay Using Gel Electrophoresis.- Transfection of Mammalian Cells In Vitro: Use in Analysis of Neuronal Damage.- Detection of Apoptotic Cells in the Nervous System.- Translocation Assays of Protein Kinase C Activation.- Astrocyte and Neuron Coculturing Method.- Viability Assays for Cells In Vitro: The Ethidium/Calcein Assay and the Immunofluorescence Combination Assay.- Measurement of the Nitric Oxide Synthase Activity Using the Citrulline Assay.- Measurement of Intraneuronal Free Calcium Using the Fluorescent Probe Technique.- Measurement of Calcium Buffering by Intracellular Organelles in Brain.- Receptor-Mediated Release of Inositolphosphates in Brain Slices.- The Use of Enzyme-Linked Immunosorbent Assays to Quantitate Proteins in Biological Functions.- Assays for the Analysis of Synaptic Proteins in Neurodegenerative Disorders.- Zymographic Method for the Measurement of Gelatinase Activity in Brain Tissue.- The Measurement of Monoamine Neurotransmitters in Microdialysis Perfusates Using HPLC-ECD.- Biochemical Measurement of Cholinesterase Activity.- Functional Study of Glutamate Receptor Channels in Brain Slices.- Selective Silver Staining for Neural Degeneration.- Immunohistological Markers of Cerebrovascular Integrity.- Electron Probe X-Ray Microanalysis: A Quantitative Electron Microscopy Technique for Measurement of Elements and Water in Nervous Tissue Cells.