Flavins and Flavoproteins

Methods and Protocols

AvMaria Barile

Häftad, Engelska, 2022

Del 2280 i serien Methods in Molecular Biology

1 273 kr

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Beskrivning

This book of protocols is devoted to the yellow coenzymes derived from riboflavin or vitamin B2 and to the hundreds of enzymes whose functionality depends on them, and represents a compendium of techniques for working with flavoproteins or with the wide spectrum of functions that flavoproteins can drive in the cells.

Produktinformation

Utgivningsdatum:2022-03-23
Mått:178 x 254 x 17 mm
Vikt:590 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:298
Förlag:Springer-Verlag New York Inc.
ISBN:9781071612880

Utforska kategorier

Kemi inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik

Innehållsförteckning

Selection of Riboflavin Overproducing Strains of Lactic Acid Bacteria and Riboflavin Direct Quantification by Fluorescence.- Recent Advances in Construction of the Efficient Producers of Riboflavin and Flavin Nucleotides (FMN, FAD) in the Yeast Candida Famata.- Overexpression of Riboflavin Excretase Enhances Riboflavin Production in the Yeast Candida Famata.- Functional Study of the Human Riboflavin Transporter 2 Using Proteoliposomes System.- Heterologous Over-Expression of Human FAD Synthase Isoforms 1 and 2.- Purification of Recombinant Human 6His-FAD Synthase (Isoform 2) and Quantitation of FAD / Protein Monomer Ratio by UV/Vis Spectra.- Continuous and Discontinuous Approaches to Study FAD Synthesis and Degradation Catalyzed by Purified Recombinant FAD Synthase or Cellular Fractions.- Redox Titration of Flavoproteins: An Overview.- Anaerobic Stopped-Flow Spectrophotometry with Photodiode Array Detection in the Pre-Steady State: An Application to Elucidate Oxido-Reduction Mechanisms in Flavoproteins.- Atomic Force Microscopy: Single Molecule Imaging and Force Spectroscopy in the Study of Flavoproteins Ligand Binding and Reaction Mechanisms.- Ligand Binding in Allosteric Flavoproteins: Part 1. Quantitative Analysis of the Interaction with NAD+ of the Apoptosis Inducing Factor (AIF) Harboring FAD in the Reduced State.- Ligand Binding in Allosteric Flavoproteins: Part 2. Quantitative Analysis of the Redox-Dependent Interaction of the Apoptosis-Inducing Factor (AIF) with Its Protein Partner.- Using D- and L-Amino Acid Oxidases to Generate the Imino Acid Substrate to Measure the Activity of the Novel Rid (Enamine/Imine Deaminase) Class of Enzymes.- The In Vitro Production of prFMN for Reconstitution of UbiD Enzymes.- Alcohol Oxidase from the Methylotrophic Yeast Ogataea polymorpha: Isolation, Purification, and Bioanalytical Application.- Flavocytochrome b2 of the Methylotrophic Yeast Ogataea polymorpha:Construction of Overproducers, Purification, and Bioanalytical Application.- Mammalian Flavoproteome Analysis Using Label-Free Quantitative Mass Spectrometry.- Alteration of Flavin Cofactor Homeostasis in Human Neuromuscular Pathologies.