Plant Lipids

Methods and Protocols

AvDorothea Bartels,Peter Dörmann

Häftad, Engelska, 2022

Del i serien Methods in Molecular Biology

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Beskrivning

This volume explores analytical methods to study complex lipid mixtures from plants and algae.

Produktinformation

Utgivningsdatum:2022-05-30
Mått:178 x 254 x 27 mm
Vikt:909 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:472
Förlag:Springer-Verlag New York Inc.
ISBN:9781071613641

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Innehållsförteckning

Lipid Isolation from Plants.- Three Methods to Extract Membrane Glycerolipids: Comparing Sensitivity to Lipase Degradation and Yield.- Thin-Layer Chromatography.- Lipid Analysis by Gas Chromatography and Gas Chromatography Mass Spectrometry.- 14C-Tracing of Lipid Metabolism.- Methods of Lipid Analyses for Microalgae: Charophytes, Eustigmatophytes, and Euglenophytes.- Direct Infusion Mass Spectrometry for Complex Lipid Analysis.- Fatty Acid Composition by Total-Acyl-Lipid--Collision Induced Dissociation--Time-of-Flight (TAL-CID-TOF) Mass Spectrometry.- Targeted Analysis of the Plant Lipidome by UPLC-NanoESI-MS/MS.- Mass Spectrometry-Based Profiling of Plant Sphingolipids from Typical and Aberrant Metabolism.- Analysis of Free and Esterified Sterol Content and Composition in Seeds using GC and ESI-MS/MS.- Techniques for the Measurement of Molecular Species of Acyl-CoA in Plants and Microalgae.- Quantification of Acyl-Acyl Carrier Proteins for Fatty Acid Synthesis using LC-MS/MS.- Structural Analysis of Glycosylglycerolipids using NMR Spectroscopy.- Analysis of Extracellular Cell Wall Lipids: Wax, Cutin and Suberin in Leaves, Roots, Fruits, and Seeds.- Isolation of Lipid Droplets for Protein and Lipid Analysis.- Isolation of Plastoglobules for Lipid Analyses.- Isolation of Mitochondria for Lipid Analysis.- Collection and Analysis of Phloem Lipids.- Analyses of Inositol Phosphates and Phosphoinositides by Strong Anion Exchange (SAX)-HPLC.- Analysis of Phosphoinositides from Complex Plant Samples by Solid-Phase Adsorption Chromatography and Subsequent Quantification via Thin-Layer and Gas Chromatography.- Studying Lipid-Protein Interactions Using Protein-Lipid-Overlay and Protein-Liposome-Association Assays.- Investigations of Lipid Binding to Acyl-CoA-Binding Proteins (ACBP) Using Isothermal Titration Calorimetry (ITC).- In Situ Localization of Plant Lipid Metabolites by Matrix Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging (MALDI-MSI).- Plant Lipid Databases.- Lipid Pathway Databases with a Focus on Algae.