This second edition brings together a variety of method used in the field of cardiovascular development research. Chapters guide readers through protocols on lymphatic development, lymphatic staining techniques, lymphatic endothelial, tissue clearing techniques, and muscle cell isolation and culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfallsAuthoritative and practical, Cardiovascular Development: Methods and Protocols, Second Edition seeks to aid scientists in understanding new state-of-the-art techniques in the field of cardiovascular development research.

Produktinformation

Utgivningsdatum:2021-07-31
Mått:178 x 254 x 16 mm
Vikt:533 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:162
Upplaga:2
Förlag:Springer-Verlag New York Inc.
ISBN:9781071614792

Utforska kategorier

Innehållsförteckning

Tissue clearing and 3-D visualization of Vasculature with the PEGASOS Method.- Preparation and Identification of Cardiac Myofibrils from Whole Heart Sample.- RNA-Sequencing and Transcriptome Analysis after Fluid Shear Stress Stimulation in Lymphatic Endothelial Cells.- Preparation of Neonatal Rat Papillary Muscles for Contractile Studies.- In Vitro Study of permeability in Lymphatic Endothelial Cells Responding to Histamine.- Optimizing the Differentiation of Cardiomyocytes from Human Induced Pluripotent Derived-Stem Cells.- Analysis of Lymphatic Vessel Formation by Whole-mount Immunofluorescence Staining.- Isolation and Culture of Mouse Lymphatic Endothelial Cells from Lung Tissue.- Laser-induced Choroidal Neovascularization in Rats.- Visualizing Three-Dimensional Vascular Networks in the Mouse Embryonic Hindbrain.- Visualizing Blood Vessel Development in Cultured Mouse Embryos Using Lightsheet Microscopy.- Laser Capture Microdissection of Vascular Endothelial Cells from Frozen Heart Tissues.- Visualization of Fundus Blood Vessels.- Transient Transgenics: An Efficient Method to Identify Gene Regulatory Elements.- Isolation of Lymphatic Muscle Cells (LMCs) from Rat Mesentery.- Isolation of Adult Mouse Cardiomyocytes using Langendorff Perfusion Apparatus.- Visualization of Angiogenesis in Mouse Embryonic Dorsal Skin by Whole-Mount Fluorescent Staining.

Mer från samma författare

Cardiovascular Development

Xu Peng, Marc Antonyak

Inbunden, 2012

1 135 kr

Cardiovascular Development

Marc Antonyak, Xu Peng

E-bok

2012

1 470 kr

Weeding of Academic Library Collections - A Case Study of UIBE University Library

Xiaohang Qi, Xu Peng, Yinyin Xu

Häftad, 2022

216 kr

Cardiovascular Development

Xu Peng, Marc Antonyak

Häftad, 2016

2 109 kr

Mer från samma serie

Del 2834

Computational Toxicology

Orazio Nicolotti

Inbunden, 2024

2 041 kr

Del 2996

Biofuels from Algae

Kristian Spilling

Inbunden, 2026

2 202 kr

Adenovirus

Miguel Chillón, Assumpció Bosch

Häftad, 2016

1 191 kr

Ctenophores

Leonid L. Moroz

Häftad, 2025

1 982 kr

Del 2588

Oral Biology

Gregory J. Seymour, Mary P. Cullinan, Nicholas C.K. Heng, Paul R. Cooper

Häftad, 2023

2 267 kr

Proteins

John M. Walker

Inbunden, 1984

1 104 kr

Nucleic Acids

John M. Walker

Inbunden, 1984

1 137 kr

Proteins

John M. Walker

Häftad, 1984

1 104 kr

Nucleic Acids

John M. Walker

Häftad, 1984

1 104 kr

New Protein Techniques

John M. Walker

Inbunden, 1988

1 104 kr

Du kanske också är intresserad av

10% rabatt på allt

L'amour toujours : Berättelsen om Tidö

Maggie Strömberg, Torbjörn Nilsson

Inbunden, 2026

275 kr

Del 2319

Cardiovascular Development

Xu Peng, Warren E. Zimmer

Häftad, 2022

1 250 kr

Cardiovascular Development

Warren E. Zimmer, Xu Peng

E-bok

2021

1 622 kr

Del 2319

Down Girl

Kate Manne

Häftad, 2020

322 kr

Del 2319

Der Einfluß der Elektronen-Turbulenz auf die elektrische Mikrofeldverteilung im Plasma

Günter Ecker

Häftad, 1973

570 kr

Del 2319

Strategisches Management, Kooperation Und Der Einfluß Von Informations- Und Kommunikationstechnologien

Oliver Motz

Häftad, 1998

759 kr

Del 2319

Convex Cones

Rolf Schneider

Häftad, 2022

334 kr

Del 2319

Uebergang Stiller Reserven Im Steuerrecht Der Kapitalgesellschaft

Isabel Reinhardt

Häftad, 1998

678 kr

Del 2319

HCI International 2024 – Late Breaking Posters

Constantine Stephanidis, Margherita Antona, Stavroula Ntoa, Gavriel Salvendy

Häftad, 2024

1 586 kr

Cardiovascular Development

Xu Peng, Marc Antonyak

Inbunden, 2012

1 135 kr

Cardiovascular Development

Methods and Protocols

AvXu Peng,Warren E. Zimmer

Inbunden, Engelska, 2021

Del 2319 i serien Methods in Molecular Biology

1 702 kr

Beställningsvara. Skickas inom 10-15 vardagar. Fri frakt över 249 kr.

Fler format och utgåvor

1 615 kr

1 585 kr

1 622 kr

1 250 kr

Beskrivning

Produktinformation

Utgivningsdatum:2021-07-31
Mått:178 x 254 x 16 mm
Vikt:533 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:162
Upplaga:2
Förlag:Springer-Verlag New York Inc.
ISBN:9781071614792

Utforska kategorier

Innehållsförteckning

Tissue clearing and 3-D visualization of Vasculature with the PEGASOS Method.- Preparation and Identification of Cardiac Myofibrils from Whole Heart Sample.- RNA-Sequencing and Transcriptome Analysis after Fluid Shear Stress Stimulation in Lymphatic Endothelial Cells.- Preparation of Neonatal Rat Papillary Muscles for Contractile Studies.- In Vitro Study of permeability in Lymphatic Endothelial Cells Responding to Histamine.- Optimizing the Differentiation of Cardiomyocytes from Human Induced Pluripotent Derived-Stem Cells.- Analysis of Lymphatic Vessel Formation by Whole-mount Immunofluorescence Staining.- Isolation and Culture of Mouse Lymphatic Endothelial Cells from Lung Tissue.- Laser-induced Choroidal Neovascularization in Rats.- Visualizing Three-Dimensional Vascular Networks in the Mouse Embryonic Hindbrain.- Visualizing Blood Vessel Development in Cultured Mouse Embryos Using Lightsheet Microscopy.- Laser Capture Microdissection of Vascular Endothelial Cells from Frozen Heart Tissues.- Visualization of Fundus Blood Vessels.- Transient Transgenics: An Efficient Method to Identify Gene Regulatory Elements.- Isolation of Lymphatic Muscle Cells (LMCs) from Rat Mesentery.- Isolation of Adult Mouse Cardiomyocytes using Langendorff Perfusion Apparatus.- Visualization of Angiogenesis in Mouse Embryonic Dorsal Skin by Whole-Mount Fluorescent Staining.