Permeability Barrier

Methods and Protocols

AvKursad Turksen

Inbunden, Engelska, 2021

Del i serien Methods in Molecular Biology

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Beskrivning

This detailed edition reflects the significant new findings in the components of permeability barriers and how they work in different tissues with a collection of cutting-edge techniques.

Produktinformation

Utgivningsdatum:2021-07-01
Mått:178 x 254 x 24 mm
Vikt:838 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:325
Upplaga:2
Förlag:Springer-Verlag New York Inc.
ISBN:9781071616727

Utforska kategorier

Cellbiologi inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik

Innehållsförteckning

- Use of Ussing Chambers to Measure Paracellular Permeability to Macromolecules in Mouse Intestine. - Rapid Evaluation of Intestinal Paracellular Permeability Using the Human Enterocytic-Like Caco-2/TC7 Cell Line. - Evaluation of Barrier Functions in Human iPSC-Derived Intestinal Epithelium. - Selective Regional Isolation of Brain Microvessels. - Estimating Brain Permeability Using In Vitro Blood-Brain Barrier Models. - In Vitro Human Blood-Brain Barrier Model for Drug Permeability Testing. - Evaluation of Blood-Brain Barrier Integrity Using Vascular Permeability Markers: Evans Blue, Sodium Fluorescein, Albumin-Alexa Fluor Conjugates, and Horseradish Peroxidase. - Blood-Brain Barrier (BBB) Permeability and Transport Measurement In Vitro and In Vivo. - Assessment of Blood-Brain Barrier Permeability Using Miniaturized Fluorescence Microscopyin Freely Moving Rats. - Measurement of Lung Vessel and Epithelial Permeability In Vivo with Evans Blue. - Measurement of Airway Epithelial Permeability: Methods and Protocols. - Vascular Permeability Assays In Vivo. - Endothelial Permeability Assays In Vitro. - Mapping Receptor Antibody Endocytosis and Trafficking in Brain Endothelial Cells. - An In Vitro Assay to Monitor Sertoli Cell Blood-Testis Barrier (BTB) Integrity. - Ussing Chamber Methods to Study the Esophageal Epithelial Barrier. - A Simple Method to Test Mechanical Strain on Epithelial Cell Monolayers Using a 3D-Printed Stretcher. - Differentiating Between Tight Junction-Dependent and Tight Junction-Independent Intestinal Barrier Loss In Vivo. - Establishment of Intestinal Epithelial Cell Monolayers and Their Use in Calcium Switch Assay for Assessment of Intestinal Tight Junction Assembly. - A Method to Prepare a Bioprobe for Regulatory Science of the Drug Delivery System to the Brain: An Angulin Binder to Modulate Tricellular Tight Junction-Seal. - dSTORM Imaging and Analysis of Desmosome Architecture. - Correction to: Differentiating Between Tight Junction-Dependent and Tight Junction-Independent Intestinal Barrier Loss In Vivo.