PCR Primer Design

Inbunden, Engelska, 2021

Del i serien Methods in Molecular Biology

2 206 kr

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Beskrivning

Chapters are divided into seven parts, including primer design strategies for quantitative PCR, genotyping, multiplex PCR, in silico PCR primer design, and primer design to identify plant and animal viruses.

Produktinformation

Utgivningsdatum:2021-11-14
Mått:178 x 254 x 22 mm
Vikt:751 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:276
Upplaga:3
Förlag:Springer-Verlag New York Inc.
ISBN:9781071617984

Utforska kategorier

Medicinsk genetik inom Medicin

Innehållsförteckning

The significance of PCR primer design in genetic diversity studies; exemplified by recent research into the genetic structure of marine species.- Enhancing cohort PASA efficiency from lessons assimilated by mutant genotyping in C. elegans.- Design of oligonucleotides for allele-specific amplification based on PCR and isothermal techniques.- Detection of rubella virus by tri-primer RT-PCR assay and genotyping by fragment RT-PCR.- Design of mismatch primers to identify and differentiate closely related (sub)species – application to the authentication of meat products.- Primer design for the analysis of closely related species – application of non-coding mtDNA and cpDNA sequences.- Designing PCR primers for the amplification-refractory mutation system.- Validation of circular RNAs by PCR.- Primer Designing for Amplifying an AT-Rich Promoter from Arabidopsis thaliana.- PLASmid TAXonomic PCR (PlasTax-PCR), a multiplex relaxase MOB typing to assort plasmids into taxonomic units.- Multiplex PCR Design for Scalable Resequencing.- Identification of gene copy number in the transgenic plants by quantitative polymerase chain reaction (qPCR).- qPrimerDB: A powerful and user-friendly database for qPCR primer design.- PCR primer design for the rapidly evolving SARS-CoV-2 genome.- Universal primers for detection of novel plant capsid-less viruses: Papaya umbra-like viruses as example.- A guide to using FASTPCR software for PCR, in silico PCR, and oligonucleotide analysis.- Pyrosequencing Primer Design for Forensic Biology Applications.-Phosphate‌ ‌methylated‌ ‌oligonucleotides‌ ‌as‌ ‌a‌ ‌novel‌ ‌primer‌ ‌for‌ ‌PCR‌ ‌and‌ ‌RT-PCR‌.