Bacterial Vaccines

Methods and Protocols

AvFadil Bidmos,Janine Bossé

Häftad, Engelska, 2022

Del i serien Methods in Molecular Biology

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Beskrivning

This volume will cover methods developed in the last decade, the usage of which are enabling the development of cheaper, cost-effective and structurally stable vaccines for global use.

Produktinformation

Utgivningsdatum:2022-11-18
Mått:178 x 254 x 26 mm
Vikt:873 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:453
Förlag:Springer-Verlag New York Inc.
ISBN:9781071619025

Utforska kategorier

Infektionssjukdomar och smittsamma sjukdomar inom Medicin
Patologi inom Medicin
Samhällsmedicin och preventiv medicin inom Medicin

Innehållsförteckning

Vaccine Design by Reverse Vaccinology and Machine Learning.- Application of Reverse Vaccinology and Immunoinformatic Strategies for the Identification of Vaccine Candidates against Shigella flexneri.- Purification of Prospective Vaccine Antigens from Gram-Positive Pathogens by Immunoprecipitation.- Rapid Surface Shaving for Proteomic Identification of Novel Surface Antigens for Vaccine Development.- Two-Dimensional Electrophoresis Coupled with Western Blot as a Method to Detect Potential Neutralizing Antibody Targets from Gram-Negative Intracellular Bacteria.- Panproteome Analysis of the Human Antibody Response to Bacterial Vaccines and Challenge.- Low-Energy Electron Irradiation (LEEI) for the Generation of Inactivated Bacterial Vaccines.- Design and Production of Hybrid Antigens for Targeting Integral Outer Membrane Proteins in Gram-Negative Bacteria.- Preparation of Trimethyl Chitosan-Based Polyelectrolyte Complexes for Peptide Subunit VaccineDelivery.- Multiepitope Fusion Antigen - MEFA, An Epitope- and Structure-Based Vaccinology Platform for Multivalent Vaccine Development.- Production, Isolation, and Characterization of Bioengineered Bacterial Extracellular Membrane Vesicles Derived from Bacteroides thetaiotaomicron and Their Use in Vaccine Development.- Membrane Vesicles Produced by Shewanella vesiculosa HM13 as a Prospective Platform for Secretory Production of Heterologous Proteins at Low Temperatures.- Glycine Induction Method: Effective Production of Immunoactive Bacterial Membrane Vesicles with Low Endotoxin Content.- Methods for Assessment of OMV/GMMA Quality and Stability.- Production of Vaccines using Biological Conjugation.- Immunological Assessment of Lung Responses to Inhalational Lipoprotein Vaccines against Bacterial Pathogens.- Determination of Maternal and Infant Immune Responses to Pertussis Vaccination in Pregnancy.- Generation of a Universal Human Complement Source by Large-Scale Depletion of IgG and IgM from Pooled Human Plasma.- Assessment of Serum Bactericidal and Opsonophagocytic Activity of Antibodies to Gonococcal Vaccine Targets.- Opsonophagocytic Killing Assay to Measure Anti-Group A Streptococcus Antibody Functionality in Human Serum.- Neisseria lactamica Controlled Human Infection Model.- Analyzing Macrophage Infection at the Organ Level.- Multi-Color Flow Cytometry and High-Dimensional Data Analysis to Probe Complex Questions in Vaccinology.