Cell Polarity Signaling

Methods and Protocols

AvChenbei Chang,Jianbo Wang

Häftad, Engelska, 2022

Del i serien Methods in Molecular Biology

1 800 kr

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Beskrivning

Beginning with advanced imaging and biochemical methods, the book continues with planar cell polarity (PCP) signaling in morphogenesis in diverse developmental contexts, apicobasal (AB) cell polarity in development and diseases, as well as directional cell migration and biomechanics in cell polarity.

Produktinformation

Utgivningsdatum:2022-02-11
Mått:178 x 254 x 30 mm
Vikt:1 011 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:530
Förlag:Springer-Verlag New York Inc.
ISBN:9781071620373

Utforska kategorier

Cellbiologi inom Naturvetenskap och teknik

Innehållsförteckning

Use of Fluorescence Recovery after Photobleaching (FRAP) to Measure In Vivo Dynamics of Cell Junction-Associated Polarity Proteins.- FRET Imaging of Rho GTPase Activity with Red Fluorescent Protein-Based FRET Pairs.- Live‐Cell Total Internal Reflection Fluorescence (TIRF) Microscopy to Investigate Protein Internalization Dynamics.- Single-Cell Single Molecule Pull-Down (sc-SiMPull) for Detection of Protein Complexes from Embryonic Lysates.- Biochemical Assays to Detect Activation of Small GTPases Rho, Rac, and Cdc42 during Morphogenesis.- Analysis of Planar Cell Polarity Complexes by Proximity Biotinylation in Xenopus Embryos.- Regulation of Cell Polarity by Posttranslational Protein Palmitoylation.- Enrichment and Detection of Wnt Proteins from Cell Culture Media.- Using Live-Imaging to Examine Early Cardiac Development in Zebrafish.- Imaging Planar Cell Polarity Proteins in Xenopus Neuroectoderm.- In Ovo Gain- and Loss-of-Function Approaches to Study Gut Morphogenesis.- Avian Embryosas a Model to Study Vascular Development.- BAC Recombineering and Transgenesis to Study Cell Polarity and Polarized Tissue Morphogenesis in Mice.- Two-Photon Cell and Tissue Level Laser Ablation Methods to Study Morphogenetic Biomechanics.- Protocols for Investigating the Epithelial Properties of Cardiac Progenitor Cells in the Mouse Embryo.- Methods to Investigate Cell Polarity of Inner Ear.- Characterization of Axon Guidance Phenotypes in Wnt/PCP Mutant Mice.- In Vitro Explant Assays and Cultures to Study PCP Signaling in Axon Guidance.- Biochemical and Cellular Assays to Study Mechanisms of PCP Signaling in Axon Guidance.- Dissection, Fixation, and Immunostaining of the Drosophila Midgut.- In Vivo Analysis of Pathways Regulating Epithelial Polarity and Secretion Using Drosophila Salivary Glands.- Imaging Epidermal Cell Rearrangement in the C. elegans Embryo.- Methods for the Study of Apical Constriction during Ascidian Gastrulation.- Assays for Apical Constriction Using the Xenopus Model.- The Use of 3-Dimensional Cell Culture to Study Apicobasal Polarization and Lumen Formation.- Studying Cell Polarity Dynamics during Cancer Initiation Using Inducible 3D Organotypic Cultures.- Under-Agarose Chemotaxis and Migration Assays for Dictyostelium.- Mapping Asymmetry in Collective Cell Migration: Lessons from Border Cells in Drosophila Oogenesis.- Targeted Injections, Atomic Force Microscopy, and Hydrogels: A Toolbox to Study Tissue Mechanics In Vivo and Ex Vivo.- Quantitative Analysis of Directional Neural Crest Cell Migration.