Induced Pluripotent Stem (iPS) Cells

Methods and Protocols

AvAndras Nagy,Kursad Turksen

Inbunden, Engelska, 2022

Del i serien Methods in Molecular Biology

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Beskrivning

This extensive new edition presents protocols reflecting the great strides made in the study of induced pluripotent stem (iPS) cells.

Produktinformation

Utgivningsdatum:2022-06-23
Mått:178 x 254 x 52 mm
Vikt:1 782 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:835
Upplaga:2
Förlag:Springer-Verlag New York Inc.
ISBN:9781071621189

Utforska kategorier

Biologi inom Naturvetenskap och teknik

Innehållsförteckning

- Human Induced Pluripotent Stem Cell (iPSC) Handling Protocols: Maintenance, Expansion, and Cryopreservation. - Generation of Induced Pluripotent Stem Cells from Human Bone Marrow–Derived Mesenchymal Stem Cells. - Analysis of Clonal Composition in Human iPSC and ESC and Derived 2D and 3D Differentiated Cultures. - Culturing Human Pluripotent Stem Cells on Micropatterned Silicon Surfaces. - Porcine iPSC Generation: Testing Different Protocols to a Successful Application. - Efficient High-Density hiPSCs Expansion in Simple Dialysis Device. - Expanding the Differentiation Potential of Already-Established Pluripotent Stem Cells. - Generation of Quiescent Cardiac Fibroblasts Derived from Human Induced Pluripotent Stem Cells. - Inductive Coculture Differentiation of Induced Pluripotent Stem Cells into Cardiomyocytes. - 3D Microwell Platform for Cardiomyocyte Differentiation of Human Pluripotent Stem Cells. - Scalable Generation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. - Application of Human Induced Pluripotent Stem Cell Technology for Cardiovascular Regenerative Pharmacology. - Efficient and Safe Method of Generating Induced Pluripotent Stem Cells from Human Skin Fibroblasts and Subsequent Differentiation into Functional Cardiomyocytes. - An Optical-Flow-Based Method to Quantify Dynamic Behavior of Human Pluripotent Stem Cell-Derived Cardiomyocytes in Disease Modeling Platforms. - Production of Innervated Skeletal Muscle Fibers Using Human Induced Pluripotent Stem Cells. - Generation of Human Neural Progenitors from Blood Samples by Interrupted Reprogramming. - Robust and Highly Efficient Protocol for Differentiation of Human Pluripotent Stem Cells into Mesenchymal Stem Cells. - The Development of Tissue Engineering Scaffolds Using Matrix from iPS-Reprogrammed Fibroblasts. - 3D Organoid Culture Using Skin Keratinocytes Derived from Human Induced Pluripotent Stem Cells. - Pluripotent Stem Cell Differentiation Toward Functional Basal Stratified Epithelial Cells. - Serum-Free Production of Three-Dimensional Hepatospheres from Pluripotent Stem Cells. - The Differentiation of Human Induced Pluripotent Stem Cells into Podocytes In Vitro. - A Method for Encapsulation and Transplantation into Diabetic Mice of Human Induced Pluripotent Stem Cells (hiPSC)-Derived Pancreatic Progenitors. - Highly Efficient Differentiation of Human Pluripotent Stem Cells into Pancreatic Progenitors Co-expressing PDX1 and NKX6.1. - Derivation of Three-Dimensional Human Induced Pluripotent Stem Cell-Derived Vocal Fold Mucosa for Clinical and Pharmacological Applications. - Generation of Human Induced Pluripotent Stem Cells Using Endothelial Progenitor Cells Derived from Umbilical Cord Blood and Adult Peripheral Blood. - Development of a Blood–Brain Barrier Permeability Assay Using Human Induced Pluripotent Stem Cell Derived Brain Endothelial Cells. - Revised “hPSC-Sac Method” for Simple and Efficient Differentiation of Human Pluripotent Stem Cells to Hematopoietic Progenitor Cells. - Efficient Generation of iPSC-Derived Hematoendothelial Progenitors and Specification Toward T cell Lineage. - Derivation and Characterization of Mesenchymal Stem Cells from iPS Cells. - Human Pluripotent Stem Cell Differentiation to Microglia. - Human Induced Pluripotent Stem Cell-Derived Microglia (hiPSC-Microglia). - Isolation and Culture of Human-Induced Pluripotent Stem Cell-Derived Cerebral Organoid Cells. - A Simple Method for Generating, Clearing, and Imaging Pre-vascularized 3D Adipospheres Derived from Human iPS Cells. - A Method for In Vitro Fabrication of Hybrid Bone/Cartilage Tissue Using Mouse Induced Pluripotent Stem Cells. - Differentiation of Human Induced Pluripotent Stem Cells (hiPSC) into Endothelial-Type Cells and Establishment of an In Vitro Blood-Brain Barrier Model. - CRISPR/Cas9-Mediated Introduction of Specific Heterozygous Mutations in Human Induced Pluripotent Stem Cells. - CRISPR/Cas9–Mediated Gene Knockout and Knockin Human iPSCs. - Generation of Monoclonal iPSC Lines with Stable Cas9 Expression and High Cas9 Activity. - CRISPR/Cas9-Mediated Genome Editing to Generate Clonal iPSC Lines. - CRISPR/Cas9 Ribonucleoprotein Complex-Mediated Efficient B2M Knockout in Human Induced Pluripotent Stem Cells (iPSCs). - Embryonal Carcinoma and Glioblastoma Cell Lines Derived from Monkey Induced Pluripotent Stem Cells. - Methods for Isolation and Reprogramming of Various Somatic Cell Sources into iPSCs. - The Characteristics of Human iPS Cells and siRNA Transfection Under Hypoxia. - mRNA-Based Reprogramming Under Xeno-Free and Feeder-Free Conditions. - Non-modified RNA-Based Reprogramming of Human Dermal Fibroblasts into Induced Pluripotent Stem Cells. - Efficient Induction of Primate iPS Cells Using a Combination of RNA Transfection and Chemical Compounds. - Generation and Cultivation of Transgene-Free Macaque and Baboon iPSCs Under Chemically Defined Conditions. - Generation of Marmoset Monkey iPSCs with Self-Replicating VEE-mRNAs in Feeder-Free Conditions. - Differentiation of Human Induced Pluripotent Stem Cells into Definitive Endoderm Using Simple Dialysis Culture Device. - Detecting and Modulating ER Stress to Improve Generation of Induced Pluripotent Stem Cells. - Gene Editing in Human Induced Pluripotent Stem Cells Using Doxycycline-Inducible CRISPR-Cas9 System. - Derivation of Clinical-Grade Induced Pluripotent Stem Cell Lines from Erythroid Progenitor Cells in Xenofree Conditions. - Generation of Murine Induced Pluripotent Stem Cells through Transposon-Mediated Reprogramming. - Human Pluripotent Stem Cells for High-Throughput Drug Screening and Characterization of Small Molecules. - Correction to: Human Pluripotent Stem Cells for High-Throughput Drug Screening and Characterization of Small Molecules.