cAMP Signaling

Methods and Protocols

AvManuela Zaccolo

Inbunden, Engelska, 2022

Del i serien Methods in Molecular Biology

1 906 kr

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Beskrivning

This volume discusses the latest techniques used by researchers to measure cAMP activity at the cell population, whole cell, and subcellular level.

Produktinformation

Utgivningsdatum:2022-03-15
Mått:178 x 254 x 27 mm
Vikt:918 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:369
Upplaga:2
Förlag:Springer-Verlag New York Inc.
ISBN:9781071622445

Utforska kategorier

Cellbiologi inom Naturvetenskap och teknik

Innehållsförteckning

Real-Time Measurements of Intracellular cAMP Gradients using FRET-Based cAMP Nanorulers.- Assaying Protein Kinase A Activity using a FRET-Based Sensor Purified from Mammalian Cells.- MultiFRET: A Detailed Protocol for High-Throughput Multiplexed Ratiometric FRET.- Photoactivated Adenylyl Cyclases as Optogenetic Modulators of Neuronal Activity.- Imaging the cAMP Signaling Microdomain of the Primary Cilium using Targeted FRET-Based Biosensors.- Methods to Assess Phosphodiesterase and/or Adenylyl Cyclase Activity via Heterologous Expression in Fission Yeast.- Time-Domain Fluorescence Lifetime Imaging of cAMP Levels with EPAC-Based FRET Sensors.- Disruptors of AKAP-Dependent Protein-Protein Interactions.- Micro-2D Cell Culture for cAMP Measurements using FRET Reporters in Human iPSC-Derived Cardiomyocytes.- Automated Image Analysis of FRET Signals for Subcellular cAMP Quantification.- In Vivo cAMP Dynamics in Drosophila Larval Neurons.- Live Cell Imaging of Cyclic Nucleotides in Human Cardiomyocytes.- Optogenetic Control of Heart Rhythm: Lightly Guiding the Cardiac Pace.- Live Imaging of cAMP Signaling in D. discoideum Based on a Bright Bioluminescent Protein, Nano-Lantern.- Generation of Transgenic Mice Expressing Cytosolic and Targeted FRET Biosensors for cAMP and cGMP.- How to Make the CUTiest Sensor in 3 Simple Steps for Computational Pedestrians.- Ion-Channel Based Reporters for cAMP Detection.- Quantitative Phosphoproteomics to Study cAMP Signalling.- Biochemical Analysis of AKAP-Anchored PKA Signaling Complexes.- Fluorescent Translocation Reporters for Sub-Plasma Membrane cAMP Imaging.- A Live-Cell Imaging Assay for Nuclear Entry of cAMP-Dependent Protein Kinase Catalytic Subunits Stimulated by Endogenous GPCR Activation.- Measuring Spatiotemporal cAMP Dynamics within an Endogenous Signaling Compartment using FluoSTEP-ICUE.