Gene Therapy of Cancer

Methods and Protocols

AvWolfgang Walther

Häftad, Engelska, 2023

Del 2521 i serien Methods in Molecular Biology

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Beskrivning

Chapters guide readers through suicide and oncolytic gene therapy, gene replacement and gene suppression therapy, vector development and refinement, immunogene therapy, TCR and CAR engineering, tumor vaccination using DNA or RNA vaccines, and antitumoral immune stimulation at different levels.

Produktinformation

Utgivningsdatum:2023-06-25
Mått:178 x 254 x 20 mm
Vikt:670 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:342
Upplaga:3
Förlag:Springer-Verlag New York Inc.
ISBN:9781071624432

Utforska kategorier

Onkologi inom Medicin
Medicinsk genetik inom Medicin

Innehållsförteckning

Brief Introduction to Current Cancer Gene Therapy.- Minicircles for CAR T Cell Production by Sleeping Beauty Transposition - A Technological Overview.- Generation of CAR-T Cells with Sleeping Beauty Transposon Gene Transfer.- Generation of Redirected Engineered Human Chimeric Antigen Receptor (CAR) T cells.- TCR- and CAR-engineering of Primary Human T cells.- TCR Gene Therapy for Cancer.- Gene Gun Her2/neu DNA Vaccination: Evaluation of Vaccine Efficacy in a Syngeneic Her2/neu Mouse Tumor Model.- Construction of Minicircle Suicide Genes Coding for Ribosome-Inactivating Proteins.- Claudin-targeted Suicide Gene Therapy for Claudin-Overexpressing Tumor Cells by Using Modified Clostridium Perfringens Enterotoxin (CPE).- Bioengineered RNA Therapy in Patient-Derived Organoids and Xenograft Mouse Models.- Application of Transcription Factor Decoy Oligodeoxynucleotides (ODNs) for Cancer Therapy.- NK Cell Effector Functions and Bystander Tumor Cell Killing in Immunovirotherapy.- A qPCR-based Method for Quantification of Replication Competent Adenovirus (RCA) in Conditionally Replicating Oncolytic Adenoviruses.- Visualized Cancer Immunotherapy with Engineered Salmonella typhimurium.- CANCER Gene Therapy: Development and Production Of Lentiviral Vectors For Gene Therapy.- Capillary Gel Electrophoresis (CGE) for Quality Control of Plasmid DNA in Gene Therapy: quality control of 20-years stored GMP-grade Plasmid DNA.- Efficient Generation of Superior Dumbbell-Shaped Non-viral DNA Delivery Vectors Using 1-2-3 Gap-primer PCR.