Apoptosis and Cancer

Methods and Protocols

AvHugo Barcenilla,David Diaz

Inbunden, Engelska, 2022

Del i serien Methods in Molecular Biology

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Beskrivning

This volume discusses methods used in the fields of molecular and cellular biology for detecting and studying cell death, especially in cancer and cancer therapy.

Produktinformation

Utgivningsdatum:2022-09-11
Mått:178 x 254 x undefined mm
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:212
Förlag:Springer-Verlag New York Inc.
ISBN:9781071625521

Utforska kategorier

Onkologi inom Medicin
Cellbiologi inom Naturvetenskap och teknik
Medicinsk utrustning och medicinska tekniker inom Medicin

Innehållsförteckning

A Non-Destructive, Real-Time Annexin V Apoptosis Assay.- Cell Death Analysis in Cancer Spheroids from a Microfluidic Device.- Apoptosis Detection by Quantification of Cell Debris in Bright-Field Microscopy Images.- Accurate Enumeration of Apoptotic Cancer Cells Using Flow Cytometry.- Assessment of the Immune Response to Tumor Cell Apoptosis and Efferocytosis.- Quantitative Analysis of Apoptosis and Necrosis in Live Cells Using Flow Cytometry.- A Fluorescence-Microscopic System for Monitoring the Turnover of the Autophagic Substrate p62/SQSTM1.- Single-Cell Profiling of the Intrinsic Apoptotic Pathway by Mass Cytometry (CyTOF).- High-Throughput, Parallel Flow Cytometry Screening of Hundreds of Cell Surface Antigens using Fluorescent Barcoding.- Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood.- Quantification of Neutrophils Undergoing NET Formation and Distinguishing Mechanisms of Neutrophil Cell Death by Use of a High-Throughput Method.- Profiling of Protein Carbonylations in Ferroptosis by Chemical Proteomics.- Cell Cycle Analysis of ER Stress and Autophagy.- Proximity Ligation In Situ Assay to Monitor Autophagy-Related Protein Interactions and Autophagy in Cancer Cells.- Simultaneous Detection of Inflammasome Activation and Membrane Damage During Pyroptosis.- Cancer and Apoptosis.