Cartilage Tissue Engineering

Inbunden, Engelska, 2022

Del i serien Methods in Molecular Biology

2 395 kr

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Beskrivning

This detailed book brings together a collection of methodologies, from the most basic to the more complex, that provides researchers with a platform they can use to embark on a cartilage research career.

Produktinformation

Utgivningsdatum:2022-11-11
Mått:178 x 254 x 28 mm
Vikt:969 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:393
Förlag:Springer-Verlag New York Inc.
ISBN:9781071628386

Utforska kategorier

Biokemisk teknik inom Naturvetenskap och teknik
Regenerativ medicin inom Medicin

Innehållsförteckning

Cartilage Tissue Engineering: An Introduction.- Chondrocyte Isolation and Expansion.- Isolation of Chondrons from Hyaline Cartilage.- Articular Cartilage Chondroprogenitors: Isolation and Directed Differentiation.- Physioxic Culture of Chondrogenic Cells.- Isolation and In Vitro Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells.- Adipose-Derived Stromal Cells: Isolation, Expansion, and Differentiation.- Chondrogenic Differentiation of Human Induced Pluripotent Stem Cells.- The Dimethylmethylene Blue Assay (DMMB) for the Quantification of Sulphated Glycosaminoglycans.- Extracellular Vesicle Isolation and Characterization for Applications in Cartilage Tissue Engineering and Osteoarthritis Therapy.- Metabolomic Profiling to Understand Chondrocyte Metabolism.- Techniques for Visualization and Quantification of Primary Cilia in Chondrocytes.- Co-Culture of Human Articular Chondrocytes Seeded in Polyurethane Scaffolds and Human Mesenchymal Stromal Cells Encapsulated in Alginate Beads.- Isolation of Murine Articular Chondrocytes for Single Cell RNA or Bulk RNA Sequencing Analysis.- Modulation of MicroRNA Expression during In Vitro Chondrogenesis.- Purification and Isolation of Proteins from Hyaline Cartilage.- Histological Preparation and Evaluation of Cartilage Specimens.- Articular Cartilage Imaging in the Context of the Superficial Chondrocyte Spatial Organization (SCSO) as a Surrogate Marker for Functional Pathology.- Unconfined Compression Experimental Protocol for Cartilage Explants and Hydrogel Constructs: From Sample Preparation to Mechanical Characterization.- Viral Gene Delivery in Chondrocytes.- Decellularization of Articular Cartilage: A Hydrochloric Acid-Based Strategy.- Self-Assembly Culture Model for Engineering Musculoskeletal Tissues.- Osteochondral Explants Isolation and Culture under a Compression and Shear Bioreactor.- A Mouse Model of Acute Cartilage Injury and Repair.- Small-Animal Compression Models of Osteoarthritis.- Modelling and Assessing Osteoarthritis in Mice by Destabilization of the Medial Meniscus (DMM).- Collagenase-Induced Osteoarthritis in Mice.- Platelet-Rich Plasma for Intra-Articular Injections: Preclinical and Clinical Evidence.